The replicative helicase MCM recruits cohesin acetyltransferase ESCO2 to mediate centromeric sister chromatid cohesion

Ivanov Miroslav P.; Ladurner Rene; Poser InaBeveridge RebeccaRampler EvelynHudecz OttoNovatchkova MariaHeriche Jean-KarimWutz Gordanavan der Lelij PetraKreidl EmanuelHutchins James R. A.Axelsson-Ekker HeinzEllenberg JanHyman Anthony A.Mechtler KarlPeters Jan-Michael

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The replicative helicase MCM recruits cohesin acetyltransferase ESCO2 to mediate centromeric sister chromatid cohesion

机译：复制的解旋酶cohesin MCM新兵乙酰转移酶ESCO2调解着丝粒姐妹染色单体的凝聚力

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Chromosome segregation depends on sister chromatid cohesion which is established by cohesin during DNA replication. Cohesive cohesin complexes become acetylated to prevent their precocious release by WAPL before cells have reached mitosis. To obtain insight into how DNA replication, cohesion establishment and cohesin acetylation are coordinated, we analysed the interaction partners of 55 human proteins implicated in these processes by mass spectrometry. This proteomic screen revealed that on chromatin the cohesin acetyltransferase ESCO2 associates with the MCM2-7 subcomplex of the replicative Cdc45-MCM-GINS helicase. The analysis of ESCO2 mutants defective in MCM binding indicates that these interactions are required for proper recruitment of ESCO2 to chromatin, cohesin acetylation during DNA replication, and centromeric cohesion. We propose that MCM binding enables ESCO2 to travel with replisomes to acetylate cohesive cohesin complexes in the vicinity of replication forks so that these complexes can be protected from precocious release by WAPL. Our results also indicate that ESCO1 and ESCO2 have distinct functions in maintaining cohesion between chromosome arms and centromeres, respectively.

机译：染色体隔离取决于姐妹染色单体期间由cohesin凝聚力DNA复制。成为阻止他们早熟的乙酰化发布之前WAPL细胞已经达到有丝分裂。复制、凝聚力建立和cohesin乙酰化作用是协调的,我们分析了人类蛋白质交互合作伙伴55参与这些过程的质量谱分析。在染色质cohesin乙酰转移酶ESCO2同事的MCM2-7复形复制Cdc45-MCM-GINS解旋酶。MCM ESCO2突变体缺陷的绑定表明,这些交互是必需的适当的招聘ESCO2染色质,在DNA复制cohesin乙酰化作用,着丝粒凝聚力。使ESCO2与replisomes旅行乙醯化凝聚力cohesin复合物附近的复制叉,这样这些配合物可以防止早熟由WAPL释放。ESCO1和ESCO2具有不同的功能保持胳膊和染色体之间的凝聚力着丝粒,分别。

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来源
《The EMBO journal.》 |2018年第15期|e97150-e97150|共21页
作者
Ivanov Miroslav P.; Ladurner Rene; Poser InaBeveridge RebeccaRampler EvelynHudecz OttoNovatchkova MariaHeriche Jean-KarimWutz Gordanavan der Lelij PetraKreidl EmanuelHutchins James R. A.Axelsson-Ekker HeinzEllenberg JanHyman Anthony A.Mechtler KarlPeters Jan-Michael;
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作者单位

European Mol Biol Lab, Heidelberg, Germany;

Res Inst Mol Pathol, Vienna, Austria;

Max Planck Inst Mol Cell Biol & Genet, Dresden, GermanyInst Mol Biotechnol, Vienna, AustriaVienna Bioctr Core Facil, NGS Facil, Vienna, Austria;

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cohesins; DNA Replication; Mitotic Sister Chromatid CohesionreplisomeCohesionReplication forkseconomic and social cohesionACETYLATIONSister Chromatid;

机译：内聚蛋白;DNA复制,有丝分裂姐妹染色单体CohesionreplisomeCohesionReplicationforkseconomic和社会cohesionACETYLATIONSister染色单体;

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1. Dpb11 Protein Helps Control Assembly of the Cdc45·Mcm2-7·GINS Replication Fork Helicase [O] . Nalini Dhingra, Irina Bruck, Skye Smith, 2015

机译：DPB11蛋白有助于控制CDC45·MCM2-7·GINS Replication Fork Helicase的组装

The replicative helicase MCM recruits cohesin acetyltransferase ESCO2 to mediate centromeric sister chromatid cohesion

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