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首页> 外文期刊>Hepatology research: the official journal of the Japan Society of Hepatology >Fas-ligand and perforin expression in infiltrating cytotoxic T lymphocytes in the liver of chronic hepatitis C.
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Fas-ligand and perforin expression in infiltrating cytotoxic T lymphocytes in the liver of chronic hepatitis C.

机译：在浸润Fas-ligand和穿孔素表达细胞毒性T淋巴细胞在肝脏慢性丙型肝炎。

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摘要

Cytotoxic T cells (CTLs) are believed to play an important role in the pathogenesis of chronic hepatitis C based on histological findings of the liver and in vivo experiments. Fas-ligand-Fas and perforin dependent pathways are two major killing systems when CTLs induce their target-cell death. Thus, the present study attempts to determine whether these pathways are activated, and if they are, how they are related in chronic hepatitis C. To investigate the expression of Fas-ligand and perforin, we performed double immunofluorescent staining of liver biopsy specimens from patients with chronic hepatitis C. Fas-ligand and perforin expression was observed in mononuclear cells, and the partial coexistence of the two proteins was observed. Cells expressing both proteins were positive for CD45RO(+) T cells (active T cells), whereas cells expressing perforin were negative for CD68 (macrophages). In the cases which had sustained negative HCV-RNA over 6 months after interferon treatment, Fas-ligand was not expressed, although perforin was slightly detectable. To quantitatively assess the balance of these pathways, hepatic mRNAs of Fas-ligand and perforin were measured by quantitative RT-PCR. The ratio of Fas-ligand-mRNA/perforin-mRNA was significantly correlated with serum alanine aminotransferase (ALT) levels (r=0.913). These results suggest that both pathways are activated in chronic hepatitis C and that Fas-ligand-Fas pathway may be predominant in active inflammation.
机译：细胞毒性T细胞(ctl)被认为发挥慢性发病机制的重要作用根据组织学的研究丙型肝炎肝脏和体内实验。穿孔素依赖的途径有两个主要的杀戮系统当ctl诱导靶细胞死亡。因此,本研究试图确定是否这些通路被激活,如果他们是,他们是如何在慢性丙型肝炎有关。调查Fas-ligand和的表达穿孔素,我们双immunofluorescent执行染色肝活检标本的病人慢性肝炎c . Fas-ligand和穿孔素在单核细胞表达观察,两种蛋白质的部分共存观察到。CD45RO阳性(+)T细胞(激活T细胞),而细胞表达穿孔素是负的CD68(巨噬细胞)。持续的消极HCV-RNA超过6个月后干扰素治疗,Fas-ligand不是表示,尽管穿孔素略可检测。这些途径,肝Fas-ligand mrna和穿孔素被定量测量rt - pcr。Fas-ligand-mRNA / perforin-mRNA明显与血清丙氨酸转氨酶(ALT)水平(r = 0.913)。这两个通路被激活在慢性丙型肝炎,Fas-ligand-Fas通路主要在活跃的炎症。

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