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首页> 外文期刊>EMBO Journal >An intragenic methylated region in the imprinted Igf2 gene augments transcription
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An intragenic methylated region in the imprinted Igf2 gene augments transcription

机译：印迹基因内的甲基化区域Igf2基因转录增加

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摘要

DNA methylation is usually associated with transcriptional silencing, but in the imprinted mouse Igf2 gene, the paternally expressed copy is methylated in two discrete differentially methylated regions (DMRs). DMR1 is located upstream of the fetal promoters and has been shown to be a methylation sensitive silencer. Here we examine the role of the intragenic DMR2 by gene targeting. In contrast to DMR1, deletion of DMR2 on the maternal allele did not lead to activation of the silent Igf2 gene. Deletion of a 54 bp methylated core region in DMR2 on the paternal allele, however, reduced Igf2 mRNA levels and was associated with fetal growth retardation. Nuclear run-on assays showed that the core region influenced transcription initiation, and luciferase reporter assays suggested that its methylation increases transcription. These results reveal a novel mechanism of gene expression whereby intragenic methylation can increase levels of transcription.
机译：DNA甲基化通常是联系在一起的转录沉默,但在印鼠标Igf2基因表达的父亲一般地复制在两个离散差异甲基化甲基化区域(dmr)。上游的胎儿的推动者,甲基化敏感的消音器。这里我们研究基因内DMR2的角色通过基因打靶。DMR2的孕产妇等位基因并没有导致激活Igf2基因的沉默。54岁的英国石油公司在DMR2甲基化核心区域然而,父亲的等位基因减少Igf2信使rna水平,与胎儿生长有关缺陷。影响转录的核心区域起始,荧光素酶记者化验建议增加其甲基化转录。的基因表达,基因内的机制甲基化可以增加的转录水平。

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