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首页> 外文期刊>EMBO Journal >Product analysis illuminates the final steps of IES deletion in Tetrahymena thermophila.
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Product analysis illuminates the final steps of IES deletion in Tetrahymena thermophila.

机译：产品分析的最后步骤在四膜虫IES删除thermophila。

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摘要

DNA sequences (IES elements) eliminated from the developing macronucleus in the ciliate Tetrahymena thermophila are released as linear fragments, which have now been detected and isolated. A PCR-mediated examination of fragment end structures reveals three types of strand scission events, reflecting three steps in the deletion process. New evidence is provided for two steps proposed previously: an initiating double-stranded cleavage, and strand transfer to create a branched deletion intermediate. The fragment ends provide evidence for a previously uncharacterized third step: the branched DNA strand is cleaved at one of several defined sites located within 15-16 nucleotides of the IES boundary, liberating the deleted DNA in a linear form.
机译：DNA序列(IES元素)的淘汰发展大核的纤毛虫四膜虫thermophila发布为线性的现在已经发现和碎片孤立。最终揭示了三种类型的链结构断开事件,反映出三个步骤删除过程。两个步骤之前提出:一个启动双链乳沟,链转移创建一个分支删除中间。片段结束之前提供依据但一个个第三步:支DNA链裂解在几个定义网站之一位于15 - 16岁的核苷酸在一个线性边界,解放被删除的DNA的形式。

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