...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>EMBO Journal >Effects of protein stability and structure on substrate processing by the ClpXP unfolding and degradation machine.
【24h】

Effects of protein stability and structure on substrate processing by the ClpXP unfolding and degradation machine.

机译：蛋白质的稳定性和结构的影响ClpXP展开和基质处理退化的机器。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

ClpXP is an ATP-dependent protease that denatures native proteins and translocates the denatured polypeptide into an interior peptidase chamber for degradation. To address the mechanism of these processes, Arc repressor variants with dramatically different stabilities and unfolding half-lives varying from months to seconds were targeted to ClpXP by addition of the ssrA degradation tag. Remarkably, ClpXP degraded each variant at a very similar rate and hydrolyzed approximately 150 molecules of ATP for each molecule of substrate degraded. The hyperstable substrates did, however, slow the ClpXP ATPase cycle. These results confirm that ClpXP uses an active mechanism to denature its substrates, probably one that applies mechanical force to the native structure. Furthermore, the data suggest that denaturation is inherently inefficient or that significant levels of ATP hydrolysis are required for other reaction steps. ClpXP degraded disulfide-cross-linked dimers efficiently, even when just one subunit contained an ssrA tag. This result indicates that the pore through which denatured proteins enter the proteolytic chamber must be large enough to accommodate simultaneous passage of two or three polypeptide chains.
机译：ClpXP变性是一个ATP-dependent蛋白酶本机蛋白质和把变性多肽为内肽酶退化。这些过程,电弧抑制因子变异显著不同的稳定性和展开从月秒半衰期不同针对ClpXP ssrA之外的降解标签。变异率和水解非常相似大约150个分子ATP基质降解的分子。然而,基质并减缓ClpXP atp酶周期。主动机制变性基质,可能适用的机械力本机结构。固有的低效或变性大量的ATP水解其他反应所需的步骤。disulfide-cross-linked二聚体有效当只有一个单元包含一个ssrA标签。结果表明,孔隙中变性蛋白质进入蛋白水解室同时必须大到足以容纳通过两个或三个多肽链。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号