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首页> 外文期刊>The British journal of cancer >Real-time PCR analysis of a 3895 bp mitochondrial DNA deletion in nonmelanoma skin cancer and its use as a quantitative marker for sunlight exposure in human skin.
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Real-time PCR analysis of a 3895 bp mitochondrial DNA deletion in nonmelanoma skin cancer and its use as a quantitative marker for sunlight exposure in human skin.

机译：实时PCR分析3895个基点的线粒体DNA在nonmelanoma皮肤癌和删除作为定量阳光的标志暴露在人类皮肤。

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摘要

Previous findings from our own laboratory have shown that the frequency of occurrence (i.e. the simple presence or absence) of the 3895 bp mitochondrial DNA deletion is increased with increasing sun exposure. The present study has significantly extended this work by developing, validating and then using a quantitative real-time PCR assay to investigate for the first time the actual level (as opposed to the frequency of occurrence) of the 3895 bp deletion in human skin from different sun-exposed body sites and tumours from nonmelanoma skin cancer patients. We investigated the 3895 bp deletion in 104 age-matched split human skin samples taken from various sun-exposed sites defined as usually exposed (n = 60) and occasionally exposed (n = 44) when outdoors. The results clearly show an increased level of the 3895 bp deletion with increasing sun exposure. Specifically, there was a significantly higher level of the deletion in the usually sun-exposed compared to the occasionally sun-exposed skin (P = 0.0009 for dermis, P = 0.008 for epidermis; two-tailed t-test). Our study has also extended previous findings by showing that the level of the 3895 bp deletion is significantly higher in the dermis compared with the epidermis both in the occasionally sun-exposed samples (P = 0.0143) and in the usually sun-exposed skin. (P = 0.0007).
机译：先前的发现来自我们自己的实验室表明,发生的频率(即简单的存在与否)的3895个基点线粒体DNA缺失增加了增加日晒。显著延长这项工作发展,使用定量验证,然后实时PCR试验调查第一时间(而不是实际的水平发生的频率)的3895个基点删除在人类皮肤从不同的人的身体网站和肿瘤从nonmelanoma皮肤癌病人。104年与人类皮肤样本从不同的人的网站通常定义为暴露(n = 60),偶尔暴露(n =在户外时44)。增加删除3895个基点的水平增加日晒。更高级别的删除相比通常引起)偶尔也会引起皮肤(P = 0.0009表皮真皮,P = 0.008;t检验)。研究结果表明,3895个基点的水平删除是显著的高于真皮相比之下,表皮的偶尔会引起样品(P = 0.0143)通常在人的皮肤。

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