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首页> 外文期刊>The British journal of cancer >Protein expression in experimental malignant glioma varies over time and is altered by radiotherapy treatment.
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Protein expression in experimental malignant glioma varies over time and is altered by radiotherapy treatment.

机译：蛋白表达在实验恶性的神经胶质瘤随着时间的变化而改变放疗治疗。

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摘要

Radiotherapy is one of the mainstays of glioblastoma (GBM) treatment. This study aims to investigate and characterise differences in protein expression patterns in brain tumour tissue following radiotherapy, in order to gain a more detailed understanding of the biological effects. Rat BT4C glioma cells were implanted into the brain of two groups of 12 BDIX-rats. One group received radiotherapy (12 Gy single fraction). Protein expression in normal and tumour brain tissue, collected at four different time points after irradiation, were analysed using surface enhanced laser desorption/ionisation - time of flight - mass spectrometry (SELDI-TOF-MS). Mass spectrometric data were analysed by principal component analysis (PCA) and partial least squares (PLS). Using these multivariate projection methods we detected differences between tumours and normal tissue, radiation treatment-induced changes and temporal effects. 77 peaks whose intensity significantly changed after radiotherapy were discovered. The prompt changes in the protein expression following irradiation might help elucidate biological events induced by radiation. The combination of SELDI-TOF-MS with PCA and PLS seems to be well suited for studying these changes. In a further perspective these findings may prove to be useful in the development of new GBM treatment approaches.
机译：放射治疗的支柱之一胶质母细胞瘤(GBM)治疗。调查并描述的差异蛋白质表达模式在脑瘤组织放疗后,为了获得一个更详细的了解生物效果。两组12 BDIX-rats的大脑。组接受放射治疗(12 Gy单身分数)。大脑肿瘤组织,在四个不同的收集时间点辐照后,进行分析使用表面增强激光解吸/电离-飞行时间质量光谱法(SELDI-TOF-MS)。主成分分析的数据分析(PCA)和偏最小二乘(PLS)。我们使用这些多变量投影方法检测到肿瘤和正常之间的区别组织,辐射treatment-induced和变化时间的影响。放射治疗后显著改变发现。辐照后表达可能会有帮助阐明生物辐射引起的事件。SELDI-TOF-MS PCA和请的结合似乎非常适合研究这些的变化。可能被证明是有用的在开发新的吗“绿带运动”的治疗方法。

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