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首页> 外文期刊>EMBO Journal >Amalgam is a ligand for the transmembrane receptor neurotactin and is required for neurotactin-mediated cell adhesion and axon fasciculation in Drosophila
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Amalgam is a ligand for the transmembrane receptor neurotactin and is required for neurotactin-mediated cell adhesion and axon fasciculation in Drosophila

机译：汞齐是一个跨膜受体的配体neurotactin和需要neurotactin-mediated细胞粘附和轴突束状的果蝇

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Neurotactin (NRT), a member of the cholinesterase-homologous protein family, is a heterophilic cell adhesion molecule that is required for proper axon guidance during Drosophila development. In this study, we identify amalgam (AMA), a member of the immunoglobulin superfamily, as a ligand for the NRT receptor. Using transfected Schneider 2 cells and embryonic primary cultures, we demonstrate that AMA is a secreted protein. Furthermore, AMA is necessary for NRT-expressing cells both to aggregate with themselves and to associate with embryonic primary culture cells. Aggregation assays performed with truncated NRT molecules reveal that the integrity of the cholinesterase-like extracellular domain was not required either for AMA binding or for adhesion, with only amino acids 347-482 of the extracellular domain being necessary for both activities. Moreover, the NRT cytoplasmic domain is required for NRT-mediated adhesion, although not for AMA binding. Using an ama-deficient stock, we find that ama function is not essential for viability, Pupae deficient for ama do exhibit defasciculation defects of the ocellar nerves similar to those found in nrt mutants. [References: 24]
机译：Neurotactin (NRT)的一员cholinesterase-homologous蛋白质家族,是一个异染的细胞粘附分子需要适当的轴突引导期间果蝇的发展。确定汞合金(AMA)的一员免疫球蛋白超家族的配体NRT受体。和胚胎的主要文化中,我们将演示AMA是一种分泌蛋白。对NRT-expressing细胞都有必要吗总与自己和关联胚胎原始培养细胞。化验与截断NRT分子泄露的完整性cholinesterase-like细胞外域并不是需要为AMA绑定或粘连,只有氨基酸347 - 482的细胞外的领域是必要的活动。需要NRT-mediated附着力,虽然不是因为AMA绑定。股票,我们发现ama函数不是必不可少的可行性,蛹缺乏ama做展览defasciculation眼球神经缺陷类似nrt突变体中找到。(引用:24)

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