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首页> 外文期刊>Lung cancer: Journal of the International Association for the Study of Lung Cancer >Comparative genomic hybridization array analysis and real time PCR reveals genomic alterations in squamous cell carcinomas of the lung.
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Comparative genomic hybridization array analysis and real time PCR reveals genomic alterations in squamous cell carcinomas of the lung.

机译：阵列比较基因组杂交分析和实时PCR揭示基因改变肺鳞状细胞癌。

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摘要

Genomic alterations have been identified in lung cancer tissues and reported in numerous studies. To analyze genomic aberrations in lung cancer patients, we used array comparative genomic hybridization (array CGH) in 14 squamous cell lung carcinoma (SqC) tissues. Copy number gain and loss in chromosomal regions were detected, and the corresponding genes were confirmed by real time PCR. Several frequently altered loci, including gain of 3q (36% of samples), were found. The most frequently identified losses were found at 14q32.33 (21% of samples). The relative degree of chromosomal change was analyzed using log2 ratios. High-level DNA amplifications (>0.8 log2 ratio) were detected at 20 regions in 1p, 2q, 3q, 4q, 6q, 7p, 8q, 9p, 10q, 12q, 14q and 19p. We found that the fold change levels were highest at EVI1 (3q26.2), LPP (3q27-28) and FHF-1 (3q28) gene loci. Our results show that array CGH is a useful tool for identification of gene alteration in lung cancer, and that the above-mentioned genes might represent potential candidate genes for pathogenesis and diagnosis of lung cancer.
机译：基因组的改变已确定在肺癌症组织和大量研究报道。分析基因在肺癌畸变病人,我们使用阵列比较基因组在14鳞状细胞杂交(全息阵列)肺癌(SqC)组织。和损失在染色体区域被检测到,和相应的基因被证实了实时PCR。包括增加3 q(36%的样本),发现。发现在14 q32.33样品(21%)。程度的染色体变化进行了分析log2比率。log2比率)被发现在1 p的20个地区,2问,3 q, 4, 6, 7 p, q, 8 9 p, 10 q, 12问,14问19个p。在邪恶(3 q26.2),最高的垂直距离(3 q27-28)和FHF-1(3 q28)基因位点。基因的识别是一个有用的工具吗变更的肺癌,上述基因可能代表的潜力候选基因的发病机制和诊断肺癌。

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