Differential expression and biodistribution of cytokeratin 18 and desmoplakins in non-small cell lung carcinoma subtypes.

Young GD; Winokur TS; Cerfolio RJVan Tine BAChow LTOkoh VGarver RI

首页> 外文期刊>Lung cancer: Journal of the International Association for the Study of Lung Cancer >Differential expression and biodistribution of cytokeratin 18 and desmoplakins in non-small cell lung carcinoma subtypes.

【24h】

Differential expression and biodistribution of cytokeratin 18 and desmoplakins in non-small cell lung carcinoma subtypes.

机译：和biodistribution微分表达式细胞角蛋白18和desmoplakins非小细胞肺部癌亚型。

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Adenocarcinoma (AC), squamous cell carcinoma (SCC) and adenosquamous carcinoma (ASC) of the lung are morphologically distinguished in part by cyto-architectural features. However, little is known about the relative expression and distribution of cyto-architectural proteins among AC, SCC and ASC. Initial microarray analysis revealed significant differences in expression of two cyto-architectural genes in AC, SCC and ASC. Desmoplakin (DP) 1 and 2, which link desmosomes to intermediate filaments, was strongly expressed in SCC relative to AC and ASC. Cytokeratin 18 (CK18), an intermediate filament that is commonly linked to desmoplakin, was strongly expressed in AC and ASC relative to SCC. Western blot analysis demonstrated that AC and ASC had abundant CK18 protein, whereas CK18 was weakly detected in SCC. DP 1 and 2 are strongly expressed in SCC and minimally expressed in AC and ASC. However, the ratio of one to the other is the same in SCC and AC, but DP2 is lost in ASC. Microscopic analysis with fluorescence-labeled antibodies for CK18 and DP 1 and 2 revealed abundant membrane localization of DP and minimal perinuclear localization of CK18 in SCC. In contrast, in both AC and ASC, the CK18 protein was diffusely distributed within the cytoplasm, and DP showed both membranous and cytoplasmic localization. In conclusion, the data here shows that AC, SCC and ASC each have specific patterns of DP 1 and 2 and CK18 gene expression, protein content and biodistribution.

机译：腺癌(AC),鳞状细胞癌(SCC)肺和adenosquamous癌(ASC)形态学杰出的部分cyto-architectural特性。了解相对表达和cyto-architectural蛋白质之间的分布交流、鳞状细胞癌和ASC。显示显著差异表达两个cyto-architectural基因交流,鳞状细胞癌和ASC。Desmoplakin (DP) 1和2,连接细胞桥粒中间丝,强烈的表达相对于交流和ASC在鳞状细胞癌。(CK18),通常是一个中间丝与desmoplakin强烈表达交流和ASC相对于鳞状细胞癌。证明了AC和ASC CK18丰富蛋白质,而CK18弱在鳞状细胞癌中发现。DP 1和2在鳞状细胞癌和强烈的表达最低限度地表达在交流和ASC。一个比另一个在鳞状细胞癌和相同交流,但在ASC DP2丢失。CK18和fluorescence-labeled抗体DP 1和2显示丰富的膜DP的本地化和最小的细胞核周围的本地化的CK18鳞状细胞癌。AC和ASC, CK18蛋白是广泛的分布在细胞质内,和DP显示膜和细胞质本地化。结论,这里的数据表明,AC,鳞状细胞癌ASC各有DP 1和2的特定模式CK18基因表达,蛋白质含量和biodistribution。

著录项

来源
《Lung cancer: Journal of the International Association for the Study of Lung Cancer》 |2002年第2期|133-141|共9页
作者
Young GD; Winokur TS; Cerfolio RJVan Tine BAChow LTOkoh VGarver RI;
展开▼
作者单位

harmacy.ankara.edu.tr;

Department of Medicine, University of Alabama at Birmingham, and Birmingham VAMC, 701 South 19th Street, LHRB 339, 35294, Birmingham, AL, USA;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类肿瘤学;
关键词
Desmoplakins; Cytokeratin 18; Intermediate FilamentsTFDP1 geneKALLIKREIN 7LungCarcinoma;

机译：Desmoplakins;细胞角蛋白18;中间FilamentsTFDP1 geneKALLIKREIN 7LungCarcinoma;

相似文献

外文文献
中文文献

1. Expression and Characterization of Catalytic Domain of T Cell Protein Tyrosine Phosphatase(ΔTC-PTP)——Immunohistochemical Study of ΔTC-PTP Expression in Non-small Cell Lung Carcinomas [J] . ZHU Zhi-cheng, SUN Mei, ZHANG Xing-yi, 高等学校化学研究（英文版） . 2007,第003期
2. Exposure to PM2.5 Enhances the PI3K/AKT Signaling and Malignancy of ERα Expression-dependent Non-small Cell Lung Carcinoma [J] . TIAN Lei, LI Ying, LIU Huan Liang, 生物医学与环境科学（英文版） . 2021,第004期
3. Correlation of Programmed Death Ligand-1 (PD-L1) Expression with Clinicopathological Features in Non-Small Cell Lung Carcinoma: Experience from a Tertiary Cancer Care Center in India [J] . Hafsa Saif, K. S. Rajesh Kumar, Suhail Sayeed Mufti, 癌症治疗（英文） . 2020,第003期
4. Adu; Aged;; Aged, 80 and over;; Antineoplastic Agents;; Carcinoma, Non-Small-Cell Lung;; Cisplatin;; Cohort Studies;; Deoxycytidine;; Female;; Humans;; Lung Neoplasms;; Male;; Middle Aged;; Mitomycin;; Neoadjuvant Therapy;; Neoplasm Staging;; Pneumonectomy;; Retrospective Studies;; Treatment Outcome;; Vinblastine;; *成年人;; 老年人;; 老年人, 80以上;; 抗肿瘤药;; 癌, 非小细胞肺;; 顺铂;; 队列研究;; 脱氧胞苷;; 肺肿瘤;; 肿瘤辅助疗法;; 肿瘤分期;; 肺切除术;; 回顾性研究;; 治疗结果;; 长春碱 [J] . Clinical cancer research: an official journal of the American Association for Cancer Research . 2009,第18期

机译：Adu; Aged;; Aged, 80 and over;; Antineoplastic Agents;; Carcinoma, Non-Small-Cell Lung;; Cisplatin;; Cohort Studies;; Deoxycytidine;; Female;; Humans;; Lung Neoplasms;; Male;; Middle Aged;; Mitomycin;; Neoadjuvant Therapy;; Neoplasm Staging;; Pneumonectomy;; Retrospective Studies;; Treatment Outcome;; Vinblastine;; *成年人;; 老年人;; 老年人, 80以上;; 抗肿瘤药;; 癌, 非小细胞肺;; 顺铂;; 队列研究;; 脱氧胞苷;; 肺肿瘤;; 肿瘤辅助疗法;; 肿瘤分期;; 肺切除术;; 回顾性研究;; 治疗结果;; 长春碱
5. Induction of Indoleamine 2,3-dioxygenase (IDO) Enzymatic Activity Contributes to Interferon-Gamma Induced Apoptosis and Death Receptor 5 Expression in Human Non-small Cell Lung Cancer Cells [O] . Chung, Ting Wen, Tan, Kok-Tong, Chan, Hong-Lin, 2015

机译：Induction of Indoleamine 2,3-dioxygenase (IDO) Enzymatic activity Contributes to Interferon-Gamma Induced apoptosis and Death Receptor 5 Expression in Human Non-small Cell Lung Cancer Cells

Differential expression and biodistribution of cytokeratin 18 and desmoplakins in non-small cell lung carcinoma subtypes.

摘要

著录项

相似文献

相关主题

期刊订阅