Ezrin is a cyclic AMP-dependent protein kinase anchoring protein.

Dransfield DT; Bradford AJ; Smith JMartin MRoy CMangeat PHGoldenring JR

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Ezrin is a cyclic AMP-dependent protein kinase anchoring protein.

机译：Ezrin循环AMP-dependent蛋白激酶锚定蛋白。

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cAMP-dependent protein kinase (A-kinase) anchoring proteins (AKAPs) are responsible for the subcellular sequestration of the type II A-kinase. Previously, we identified a 78 kDa AKAP which was enriched in gastric parietal cells. We have now purified the 78 kDa AKAP to homogeneity from gastric fundic mucosal supernates using type II A-kinase regulatory subunit (RII) affinity chromatography. The purified 78 kDa AKAP was recognized by monoclonal antibodies against ezrin, the canalicular actin-associated protein. Recombinant ezrin produced in either Sf9 cells or bacteria also bound RII. Recombinant radixin and moesin, ezrin-related proteins, also bound RII in blot overlay. Analysis of recombinant truncations of ezrin mapped the RII binding site to a region between amino acids 373 and 439. This region contained a 14-amino-acid amphipathic alpha-helical putative RII binding region. A synthetic peptide containing the amphipathic helical region (ezrin409-438) blocked RII binding to ezrin, but a peptide with a leucine to proline substitution at amino acid 421 failed to inhibit RII binding. In mouse fundic mucosa, RII immunoreactivity redistributed from a predominantly cytosolic location in resting parietal cells, to a canalicular pattern in mucosa from animals stimulated with gastrin. These results demonstrate that ezrin is a major AKAP in gastric parietal cells and may function to tether type II A-kinase to a region near the secretory canaliculus.

机译：cAMP-dependent蛋白激酶(激酶)锚定蛋白质(akap)负责II型的亚细胞封存一种激酶。丰富的胃壁细胞。现在已经净化78 kDa AKAP同质性从胃胃底粘膜上清液使用类型二世一种激酶调节亚基(RII)亲和力色谱法。被单克隆抗体ezrin,微管的actin-associated蛋白质。重组ezrin Sf9细胞或生产细菌还RII绑定。moesin ezrin-related蛋白质也绑定RII污点覆盖。ezrin映射RII绑定网站的一个地区373年和439年之间的氨基酸。包含一个14-amino-acid两亲的α螺旋的RII绑定。包含两性分子的合成肽螺旋区域(ezrin409 - 438)阻塞RII绑定ezrin,但是肽脯氨酸和亮氨酸替换在421未能抑制氨基酸RII绑定。免疫反应性的重新分配主要是胞质位置休息壁细胞微管的模式从动物粘膜刺激胃泌激素。这些结果说明ezrin是主要的AKAP胃壁细胞和可能的功能使II型一种激酶到附近的一个地区分泌小管。

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来源
《EMBO Journal》 |1997年第1期|35-43|共9页
作者
Dransfield DT; Bradford AJ; Smith JMartin MRoy CMangeat PHGoldenring JR;
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作者单位

Institute of Molecular Medicine and Genetics, Medical College of Georgia and the Augusta Veterans Affairs Medical Center, 30912, USA.;

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原文格式 PDF
正文语种英语
中图分类分子生物学;分子生物学;
关键词
Canalicular Pattern; Cytoskeletal Proteins; Cyclic AMP-Dependent Protein KinasesNitric Oxide Synthase 2AA Kinase Anchor ProteinsMucous MembraneezrinGastric Parietal Cells;

机译：微管的模式;细胞骨架蛋白;循环AMP-Dependent蛋白质氧化KinasesNitric合酶2 aa激酶锚ProteinsMucousMembraneezrinGastric壁细胞;

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Ezrin is a cyclic AMP-dependent protein kinase anchoring protein.

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