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首页> 外文期刊>EMBO Journal >ATP-dependent resolution of R-loops at the ColE1 replication origin by Escherichia coli RecG protein, a Holliday junction-specific helicase.
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ATP-dependent resolution of R-loops at the ColE1 replication origin by Escherichia coli RecG protein, a Holliday junction-specific helicase.

机译：ATP-dependent ColE1 R-loops的决议由大肠杆菌复制起源放射心电图蛋白质,霍利迪junction-specific解旋酶。

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摘要

The RecG protein of Escherichia coli is a DNA helicase that promotes branch migration of the Holliday junctions. We found that overproduction of RecG protein drastically decreased copy numbers of ColE1-type plasmids, which require R-loop formation between the template DNA and a primer RNA transcript (RNA II) for the initiation of replication. RecG efficiently inhibited in vitro ColE1 DNA synthesis in a reconstituted system containing RNA polymerase, RNase HI and DNA polymerase I. RecG promoted dissociation of RNA II from the R-loop in a manner that required ATP hydrolysis. These results suggest that overproduced RecG inhibits the initiation of replication by prematurely resolving the R-loops formed at the replication origin region of these plasmids with its unique helicase activity. The possibility that RecG regulates the initiation of a unique mode of DNA replication, oriC-independent constitutive stable DNA replication, by its activity in resolving R-loops is discussed.
机译：大肠杆菌的放射心电图蛋白DNA解旋酶促进迁移的分支霍利迪连接。放射心电图蛋白显著减少副本需要的数量的ColE1-type质粒R-loop模板DNA和之间的形成底漆RNA转录起始(RNA II)的复制。体外ColE1重组DNA合成系统含有RNA聚合酶、核糖核酸酶和你好DNA聚合酶i放射心电图提升的离解从R-loop RNA II的方式ATP水解。过度繁殖放射心电图抑制的起始过早地解决R-loops复制这些复制起源地区的形成质粒以其独特的解旋酶的活动。可能放射心电图调节的起始一个独特的DNA复制模式,oriC-independent本构稳定DNA在解决R-loops复制,由其活动进行了探讨。

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