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首页> 外文期刊>EMBO Journal >MUTS MEDIATES HETERODUPLEX LOOP FORMATION BY A TRANSLOCATION MECHANISM
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MUTS MEDIATES HETERODUPLEX LOOP FORMATION BY A TRANSLOCATION MECHANISM

机译：傻瓜介导HETERODUPLEX循环形成的易位机制

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摘要

Interaction of Escherichia coli MutS and MutL with heteroduplex DNA has been visualized by electron microscopy. In a reaction dependent on ATP hydrolysis, complexes between a MutS dimer and a DNA heteroduplex are converted to protein-stabilized, alpha-shaped loop structures with the mismatch in most cases located within the DNA loop, Loop formation depends on ATP hydrolysis and loop size increases linearly with time at a rate of 370 base pairs/min in phosphate buffer and about 10 000 base pairs/min in the HEPES buffer used for repair assay. These observations suggest a translocation mechanism in which a MutS dimer bound to a mismatch subsequently leaves this site by ATP-dependent tracking or unidimensional movement that is in most cases bidirectional from the mispair. In view of the bidirectional capability of the methyl-directed pathway, this reaction may play a role in determination of heteroduplex orientation. The rate of MutS-mediated DNA loop growth is enhanced by MutL, and when both proteins are present, both are found at the base of alpha-loop structures, and both can remain associated with excision intermediates produced in later stages of the reaction. [References: 31]
机译：大肠杆菌傻瓜和MutL交互heteroduplex DNA已经被电子可视化显微镜。水解,傻瓜二聚体和之间的配合物DNA heteroduplex转换为protein-stabilized alpha-shaped循环结构在大多数情况下位于与不匹配DNA循环,循环的形成取决于ATP水解和循环的大小线性增长时间370个碱基对的速度在磷酸/分钟缓冲区和大约10 000碱基对/分钟消息灵通的缓冲区用于修复试验。观察结果表明易位机制一个傻瓜二聚体绑定到一个不匹配随后由ATP-dependent离开这个网站跟踪或一维的运动大多数情况下从mispair双向。视图的双向能力methyl-directed通路,这个反应可能发挥在确定heteroduplex中的作用取向。增长是由MutL增强,当两者蛋白质的存在,都发现底部alpha-loop结构,都可以保持与切除相关中间体生产在以后的阶段的反应。

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