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首页> 外文期刊>EMBO Journal >WHY IS THE INITIATION NICK SITE OF AN AT-RICH ROLLING CIRCLE PLASMID AT THE TIP OF A GC-RICH CRUCIFORM
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WHY IS THE INITIATION NICK SITE OF AN AT-RICH ROLLING CIRCLE PLASMID AT THE TIP OF A GC-RICH CRUCIFORM

机译：为什么at富集的起始尼克网站滚圈质粒GC-RICH的一角十字形

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摘要

pT181 and other closely related rolling circle plasmids have the nicking site for initiation of replication between the arms of a GC-rich inverted repeat sequence adjacent to the binding site for the dimeric initiator protein, Replication is initiated by the initiator-induced extrusion of this sequence as a cruciform, creating a single-stranded region for nicking by the protein, Nicking is followed by assembly of the replisome without relaxation of the secondary structure, Following termination, the initiator protein is released with a short oligonucleotide attached to one subunit, which prevents it from being recycled, a necessary feature of the plasmid's replication control system, The modified initiator can cleave single-stranded substrates and can nick and relax supercoiled plasmid DNA weakly, Although it can bind to its recognition sequence in the leading strand origin, the modified protein cannot induce cruciform extrusion, and it is proposed that this inability is the key to understanding the biological rationale for having the nicking site at the tip of a cruciform: the need to provide the functional initiator with a catalytic advantage over the modified one sufficient to offset the numerical advantage and metabolic stability of the latter. [References: 33]
机译：pT181和其他滚动循环密切相关质粒已经发起的攻击网站复制的怀抱GC-rich之间反向重复序列相邻的绑定网站的蛋白质二聚的引发剂,复制是由initiator-induced发起挤压的序列作为一个十字形,创建一个单链区域为轻伤蛋白质,轻伤是紧随其后的是组装的二级的replisome没有放松结构,终止后,启动程序蛋白质是发布短的寡核苷酸附加到一个亚基,防止它被回收,必要的特征质粒的复制控制系统改性剂可以分裂单链基板,尼克和放松超螺旋质粒DNA弱,尽管它可以绑定到它前导链的识别序列起源,修改后的蛋白质不能诱导十字形挤压,提出这个问题不能是理解的关键生物的理由攻击网站在一个十字形的技巧:需要提供功能引发剂催化修改一个足够的优势偏移量数值和代谢优势后者的稳定。

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