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首页> 外文期刊>EMBO Journal >HUMAN AND XENOPUS CDNA ENCODING BUDDING YEAST CDC7-RELATED KINASES - IN VITRO PHOSPHORYLATION OF MCM SUBUNITS BY A PUTATIVE HUMAN HOMOLOGUE OF CDC7
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HUMAN AND XENOPUS CDNA ENCODING BUDDING YEAST CDC7-RELATED KINASES - IN VITRO PHOSPHORYLATION OF MCM SUBUNITS BY A PUTATIVE HUMAN HOMOLOGUE OF CDC7

机译：人类和非洲爪蟾蜍CDNA编码出芽酵母CDC7-RELATED激酶-体外磷酸化MCM的子单元由一个公认的人类的同系物CDC7

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摘要

Saccharomyces cerevisiae Cdc7 kinase is essential for initiation of DNA replication, and Hsk1, a related kinase of Schizosaccharomyces pombe, is also required for DNA replication of fission yeast cells. We report here cDNAs encoding Cdc7-related kinases from human and Xenopus (huCdc7 and xeCdc7, respectively), The cloned cDNA for huCdc7 contains an open reading frame consisting of 574 amino acids with a predicted molecular weight of 63 847 that possesses overall amino acid identity of 32% (54% including similar residues) to Cdc7 and Hsk1. huCDC7 is transcribed in the various tissues examined, but most abundantly in testis, Three transcripts of 4.4, 3.5 and 2.4 kb in length are detected, The 3.5 kb transcript is the most predominant and is expressed in all the tissues examined, A cDNA containing a 91 nucleotide insertion at the N-terminal region of huCDC7 is also detected, suggesting the presence of multiple splicing variants, The huCdc7 protein is expressed at a constant level during the mitotic cell cycle and is localized primarily in nuclei in interphase and distributed diffusibly in cytoplasm in the mitotic phase, The wild-type huCdc7 protein expressed in COS7 cells phosphorylates MCM2 and MCM3 proteins in vitro, suggesting that huCdc7 may regulate processes of DNA replication by modulating MCM functions. [References: 56]
机译：酿酒酵母Cdc7激酶是至关重要的DNA复制的起始和Hsk1粟酒裂殖酵母的相关激酶DNA复制所需的裂变酵母细胞。从人类和非洲爪蟾蜍Cdc7-related激酶(分别huCdc7和xeCdc7),克隆互补脱氧核糖核酸huCdc7包含一个开放阅读框574个氨基酸组成的预测分子量为63 847,具有整体氨基酸的32%(包括54%相似Cdc7和Hsk1残留物)。在各种组织检查,但大多数在睾丸,三个记录为4.4,3.5和2.4 kb的长度被检测到,3.5 kb成绩单是最主要的cDNA表达所有的组织检查包含91个核苷酸的插入氨基端地区huCDC7也发现,显示多个连接的存在变异,huCdc7蛋白表达在有丝分裂细胞周期和恒定的水平主要是在间期核本地化在细胞质和分布式扩散有丝分裂阶段,野生型huCdc7蛋白质表示在COS7细胞磷酸化MCM2和MCM3蛋白质体外,这表明huCdc7可以调节DNA复制的过程吗调制MCM的功能。

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