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首页> 外文期刊>EMBO Journal >SUPPRESSORS OF YCK-ENCODED YEAST CASEIN KINASE 1 DEFICIENCY DEFINE THE FOUR SUBUNITS OF A NOVEL CLATHRIN AP-LIKE COMPLEX
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SUPPRESSORS OF YCK-ENCODED YEAST CASEIN KINASE 1 DEFICIENCY DEFINE THE FOUR SUBUNITS OF A NOVEL CLATHRIN AP-LIKE COMPLEX

机译：YCK-ENCODED酵母酪蛋白激酶1的抑制缺乏定义的四个亚基的小说网格蛋白附图像复杂

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In Saccharomyces cerevisiae, the redundant YCK1 and YCK2 genes (Yeast Casein Kinase 1) are required for viability, We describe here the molecular analysis of four mutations that eliminate the requirement for Yck activity, These mutations alter proteins that resemble the four subunits of clathrin adaptors (APs), with highest sequence similarity to those of the recently identified AP-3 complex, The four yeast subunits are associated in a high-molecular-weight complex. These proteins have no essential function and are not redundant for function with other yeast AP-related proteins, Combination of suppressor mutations with a clathrin heavy chain mutation (chc1-ts) confers no synthetic growth defects, However, a yck(ts) mutation shows a strong synthetic growth defect with chc1-ts, Moreover, endocytosis of Ste3p is dramatically decreased in yck(ts) cells and is partially restored by the AP suppressor mutations, These results suggest that vesicle trafficking at the plasma membrane requires the activity of Yck protein kinases, and that the new AP-related complex may participate in this process. [References: 55]
机译：在酿酒酵母中,冗余YCK1和YCK2基因(酵母酪蛋白激酶1)所需的生存能力,我们在这里描述四个突变的分子分析消除Yck活动的要求,这些突变改变蛋白质,像四个子单元的网格蛋白适配器(APs),最高最近序列相似性的确定AP-3复杂,四个酵母子单元在高分子量相关联复杂。功能和不冗余功能其他酵母AP-related蛋白质的结合抑制基因突变与网格蛋白重链突变(chc1-ts)授予没有合成生长缺陷,然而,yck (ts)显示了一个突变合成的强劲增长与chc1-ts缺陷,此外,Ste3p内吞作用显著减少yck (ts)细胞和部分恢复了美联社抑制突变,这些结果表明,泡贩卖的质膜需要Yck的活动蛋白激酶,新的AP-related复杂的可能参与这个过程。(引用:55)

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