The distal GATA sequences of the sid1 promoter of Ustilago maydis mediate iron repression of siderophore production and interact directly with Urbs1, a GATA family transcription factor.

An ZhiQiang; Mei BaiGen; Yuan WMLeong SAAn ZQMei BG

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The distal GATA sequences of the sid1 promoter of Ustilago maydis mediate iron repression of siderophore production and interact directly with Urbs1, a GATA family transcription factor.

机译：远端叫sid1启动子的序列黑粉菌属maydis调解铁的镇压含铁细胞生产和直接与互动Urbs1,叫家族转录因子。

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摘要

The sid1 and urbs1 genes encode L-ornithine N5-oxygenase and a GATA family transcription regulator, respectively, for siderophore biosynthesis in U. maydis [U. zeae]. The basic promoter and iron-regulatory sequences of the U. zeae sid1 gene were defined by fusing restriction and Bal31 nuclease-generated deletion fragments of the promoter region with the Escherichia coli beta-glucuronidase (GUS) reporter gene. Sequences needed for iron-regulated expression of sid1 were localized to a 306 bp region mapping 2.3 and 2.6 kb upstream of the ATG. The 306 bp region contains 2 G/TGATAA sequences, consensus DNA binding sites of GATA family transcription factors. A model involving DNA looping for the regulatory action of Urbs1 on sid1 expression is discussed.

机译：sid1和urbs1基因编码L-ornithineN5-oxygenase和家庭GATA转录监管机构,分别为含铁细胞生物合成在美国maydis (U。启动子和iron-regulatory序列。菌sid1基因融合所定义的限制和Bal31 nuclease-generated删除片段启动子区域的大肠杆菌beta-glucuronidase GUS报告基因。所需iron-regulated sid1的表达局部地区306个基点映射2.3和2.6kb ATG的上游。包含2 G / TGATAA序列,共识的DNA结合位点的GATA转录的因素。监管行动Urbs1 sid1表达式进行了讨论。

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来源
《EMBO Journal》 |1997年第7期|1742-1750|共9页
作者
An ZhiQiang; Mei BaiGen; Yuan WMLeong SAAn ZQMei BG;
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作者单位

Laboratory of Analytical chemistry, Department of Chemistry, University of Helsinki/P.O.Box55, FIN-00014 University of Helsinki, Finland;

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原文格式 PDF
正文语种英语
中图分类分子生物学;
关键词
Plant pathogen; Ustilago zeae; ProductionGenetics, Molecularplant pathologyplant pathogenic fungiSiderophoresgenome analysisIronSnail Family Transcription FactorsUstilago maydisSOX Transcription FactorsTwist-Related Protein 1;

机译：植物病原体;黑粉菌属菌;ProductionGenetics,Molecularplant pathologyplant致病性fungiSiderophoresgenome analysisIronSnail家庭转录FactorsUstilago maydisSOX转录FactorsTwist-Related蛋白1;

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外文文献

1. urbs1, a gene regulating siderophore biosynthesis in Ustilago maydis, encodes a protein similar to the erythroid transcription factor GATA-1. [J] . C Voisard, J Wang, J L McEvoy, Molecular and Cellular Biology . 1993,第11期

机译：urbs1是调控乌斯拉蒂瓜（Ustilago maydis）中铁载体生物合成的基因，其编码的蛋白质类似于类红细胞转录因子GATA-1。
2. The distal GATA sequences of the sid1 promoter of Ustilago maydis mediate iron repression of siderophore production and interact directly with Urbs1 a GATA family transcription factor. [O] . Z An, B Mei, W M Yuan, 1997

机译：Ustilago的sid1启动子的远端GATA序列可能介导铁抑制铁载体的产生并直接与GATA家族转录因子Urbs1相互作用。

The distal GATA sequences of the sid1 promoter of Ustilago maydis mediate iron repression of siderophore production and interact directly with Urbs1, a GATA family transcription factor.

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