...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>EMBO Journal >ACTIVE PHOTOSYNTHESIS IN CYANOBACTERIAL MUTANTS WITH DIRECTED MODIFICATIONS IN THE LIGANDS FOR TWO IRON-SULFUR CLUSTERS IN THE PSAC PROTEIN OF PHOTOSYSTEM I
【24h】

ACTIVE PHOTOSYNTHESIS IN CYANOBACTERIAL MUTANTS WITH DIRECTED MODIFICATIONS IN THE LIGANDS FOR TWO IRON-SULFUR CLUSTERS IN THE PSAC PROTEIN OF PHOTOSYSTEM I

机译：蓝藻活跃的光合作用的突变体与配体的直接修改两个IRON-SULFUR集群的PSAC蛋白质光系统I

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The PsaC protein of the Photosystem I (PSI) complex in thylakoid membranes coordinates two [4Fe-4S] clusters, F-A and F-B. Although it is known that PsaC participates in electron transfer to ferredoxin, the pathway of electrons through this protein is unknown. To elucidate the roles of F-A and F-B, we created two site-directed mutant strains of the cyanobacterium Anabaena variabilis ATCC 29413. In one mutant, cysteine 13, a ligand for F-B was replaced by an aspartic acid (C13D); in the other mutant, cysteine 50, a ligand for F-A was modified similarly (C50D). Low-temperature electron paramagnetic resonance studies demonstrated that the C50D mutant has a normal F-B center and a modified F-A center. In contrast, the C13D strain has normal F-A, but failed to reveal any signal from F-B. Room-temperature optical studies showed that C13D has only one functional electron acceptor in PsaC, whereas two such accepters are functional in the C50D and wild-type strains. Although both mutants grow under photoautotrophic conditions, the rate of PSI-mediated electron transfer in C13D under low light levels is about half that of C50D or wild type. These data show that (i) F-B is not essential for the assembly of the PsaC protein in PSI and (ii) F-B is not absolutely required for electron transfer from the PSI reaction center to ferredoxin.
机译：光系统I PsaC蛋白(PSI)在类囊体膜两个坐标[4 fe-4s]集群,一和F-B。知道PsaC参与电子传递铁氧还蛋白,电子通过的途径这种蛋白质是未知的。层和F-B,我们创建了两个定点藻青菌突变株的淡水藻类的一种摘要写明ATCC 29413。13日的配体F-B由天冬氨酸的取代酸(C13D);配体为一修改同样(C50D)。低温电子顺磁共振研究表明,C50D突变的正常F-B中心和改性层中心。一对比,C13D应变正常,但从F-B未能透露任何信号。室温光学研究表明,C13D只有一个功能电子受体在吗PsaC,而两个这样的接受者是功能性的C50D和野生型菌株。突变体生长能光合自养的条件下,PSI-mediated电子转移的速率C13D在低光照水平的一半左右C50D或野生型。不是基本的组装PsaC吗蛋白质在PSI (2) F-B并不是绝对的电子传递所需的PSI铁氧还蛋白反应中心。

著录项

相似文献

  • 外文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号