...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>EMBO Journal >A 41 AMINO ACID MOTIF IN IMPORTIN-ALPHA CONFERS BINDING TO IMPORTIN-BETA AND HENCE TRANSIT INTO THE NUCLEUS
【24h】

A 41 AMINO ACID MOTIF IN IMPORTIN-ALPHA CONFERS BINDING TO IMPORTIN-BETA AND HENCE TRANSIT INTO THE NUCLEUS

机译：IMPORTIN-ALPHA赋予41氨基酸基序绑定IMPORTIN-BETA因此交通细胞核

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The complex of importin-alpha and -beta is essential for nuclear protein import. It binds the import substrate in the cytosol, and the resulting trimeric complex moves through the nuclear pores, probably as a single entity, Importin-alpha provides the nuclear localization signal binding site, importin-beta the site of initial docking to the pore, Here we show that the conserved, basic N-terminus of importin-alpha is sufficient for importin-beta binding and essential for protein import. The fusion product of this 41 amino acid domain to a heterologous protein is transported into the nucleus in the same way as full-length importin-alpha itself. Transport is dependent on importin-beta but competed by importin-alpha. As no additional part of importin-alpha is needed for translocation, the movement which drives the import substrate complex into the nucleus appears to be generated between importin-beta and structures of the nuclear pore. The domain that binds to importin-beta appears to confer import only, but not re-export out of the nucleus, suggesting that the return of importin-alpha into the cytoplasm is not a simple reversal of its entry.
机译：importin-alpha和β的复杂必不可少的核内蛋白导入。的进口基质细胞溶质,三聚物的复杂穿过核孔,可能是作为一个单一的实体,Importin-alpha提供核本地化信号结合位点,importin-beta的网站初始孔隙对接,我们证明守恒的,基本的importin-alpha n端importin-beta绑定和足够吗进口所必需的蛋白质。的41个氨基酸不同的域蛋白质是运入核一样全身importin-alpha本身。运输是依赖importin-beta但通过importin-alpha相互竞争。需要importin-alpha易位,运动驱动进口基质复杂的核似乎是生成的importin-beta和结构之间的核孔。importin-beta似乎只授予进口,但是不出口的核,这表明的回归importin-alpha进入细胞质不是一个简单的条目的逆转。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号