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首页> 外文期刊>EMBO Journal >ZNT-2, A MAMMALIAN PROTEIN THAT CONFERS RESISTANCE TO ZINC BY FACILITATING VESICULAR SEQUESTRATION
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ZNT-2, A MAMMALIAN PROTEIN THAT CONFERS RESISTANCE TO ZINC BY FACILITATING VESICULAR SEQUESTRATION

机译：ZNT-2,哺乳动物蛋白质,产生了耐药性锌通过促进水泡封存

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摘要

A cDNA encoding a second zinc transporter (ZnT-2) was isolated from a rat kidney cDNA expression library by complementation of a zinc-sensitive BHK cell line. The protein predicted from the open reading frame of ZnT-2 cDNA has 359 amino acids and initiates with a CTG codon. It resembles ZnT-1 (a plasma membrane protein that stimulates zinc efflux) in overall topology in that it has six membrane-spanning domains, a histidine-rich intracellular loop and a long C-terminal tail; however, the overall amino acid identity is only 26%. Unlike ZnT-1, which is in the plasma membrane and lowers cellular zinc by stimulating zinc efflux, ZnT-2 is localized on vesicles and allows the zinc-sensitive BHK cells to accumulate zinc to levels that are much higher than non-transformed cells can tolerate. Zinc was visualized within these vesicles with zinquin, a zinc-specific fluorescent probe. The intracellular compartment that accumulates zinc is acidic as revealed by staining with acridine orange or LysoTracker. Prolonged exposure of cells expressing ZnT-2 to zinc causes an accretion of intracellular vesicles. We suggest that ZnT-2 protects these cells from zinc toxicity by facilitating zinc transport into an endosomal/lysosomal compartment.
机译：cDNA编码第二个锌转运体(ZnT-2)从一只老鼠孤立肾cDNA表达吗图书馆的互补zinc-sensitive博鸿泰细胞系。开放阅读框的ZnT-2 cDNA有359个氨基酸酸和启动CTG密码子。像ZnT-1(质膜蛋白质刺激锌流出)的整体拓扑结构它有六个membrane-spanning域,富含组氨酸胞内循环和长c端尾巴;身份的只有26%。等离子体膜,降低细胞锌刺激锌流出,ZnT-2本地化囊泡,并允许zinc-sensitive博鸿泰细胞积累锌水平更高比非转换细胞可以容忍。与zinquin可视化这些囊泡内,zinc-specific荧光探针。胞内积累锌的隔间是酸性和吖啶染色所显示的吗橙色或LysoTracker。细胞表达ZnT-2锌引起细胞内囊泡的吸积。ZnT-2保护这些细胞锌毒性通过促进锌运输到一个endosomal /溶酶体舱。

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