Nuclease Degradation Analysis of DNA Nanostructures Using Gel Electrophoresis

Arun Richard Chandrasekaran; Ken Halvorsen

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Nuclease Degradation Analysis of DNA Nanostructures Using Gel Electrophoresis

机译：核酸酶降解分析的DNA纳米结构使用凝胶电泳

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Custom-built DNA nanostructures are now used in applications such as biosensing, molecular computation, biomolecular analysis, and drug delivery. While the functionality and biocompatibility of DNA makes DNA nanostructures useful in such applications,the field faces a challenge in making biostable DNA nanostructures. Being a natural material, DNA is most suited for biological applications, but is also easily degraded by nucleases. Several methods have been employed to study the nuclease degradationrates and enhancement of nuclease resistance. This protocol describes the use of gel electrophoresis to analyze the extent of nuclease degradation of DNA nanostructures and to report degradation times, kinetics of nuclease digestion, and evaluation of biostability enhancement factors.

机译：定制的DNA纳米结构中使用应用程序如若分子计算、生物分子分析和药物交付。生物相容性的DNA DNA纳米结构有用的在这样的应用程序中,面临着挑战使biostable DNA纳米结构。作为一种天然材料,DNA是最适合的生物应用程序,但是也很容易被核酸酶降解。用来研究核酸酶degradationrates和增强抗核酸酶。协议描述了使用凝胶电泳分析核酸酶降解的程度DNA纳米结构和报告退化次,核酸酶消化动力学,评价biostability增强因素。

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来源
《Current Protocols in Nucleic Acid Chemistry》 |2020年第1期|e115/1-e115/12|共12页
作者
Arun Richard Chandrasekaran; Ken Halvorsen;
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作者单位

The RNA Institute, University at Albany, State University of New York, Albany,New York;

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原文格式 PDF
正文语种英语
中图分类
关键词
Drug Liberation; Electrophoresis Gel; BIOSTABILITYDNAdegradationnucleaseNanostructureDNA Motifs;

机译：药物解放;电泳凝胶;BIOSTABILITYDN;

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Nuclease Degradation Analysis of DNA Nanostructures Using Gel Electrophoresis

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