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首页> 外文期刊>Chemical Engineering Research & Design: Transactions of the Institution of Chemical Engineers >Evaluation of a novel low-cost culture medium containing exclusively milk, potato and glycerol for microbial transglutaminase production by Streptomyces mobaraensis
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Evaluation of a novel low-cost culture medium containing exclusively milk, potato and glycerol for microbial transglutaminase production by Streptomyces mobaraensis

机译：评价一种新颖的低成本的培养基只包含牛奶,土豆和甘油微生物转谷氨酰胺酶生产

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摘要

The aim of this work was to evaluate the production of microbial transglutaminase (MTG) by Streptomyces mobaraensis in a reduced nutritional medium based on non-commercial potatoes. Initially, the production was studied comparing 3 culture media based on potato: (i) media based on acid hydrolysed potato, (ii) media based on gelified non-hydrolysed potato and (iii) media based on gelified non-hydrolysed potato supplemented with glucose (20 g/L). All media were supplemented with nutrients and inductors of MTG production (yeast extract, peptone, sodium casemate, glycerol and mineral salts such as sodium phosphate, potassium phosphate, magnesium sulphate). The best medium was gelified non-hydrolysed potato allowed to obtain up to 2.72 U/mL at 96 h. In a second study, it was proposed the substitution of all the nutrients and inductors by exclusively skim milk and glycerol. An experimental design was performed to optimize the composition of milk, potato and glycerol. The economical yield of the process was also evaluated. Fermentations were carried out and samples were taken at 72 h and 96 h. Results showed that the maximum MTG activity was obtained at 72 h. A significant effect of potato and milk on MTG activity was observed, including an effect of interaction between both compounds. Optimal conditions selected were: 600 g/L of skim milk, 40 g/L of potato and 5 g/L of glycerol, yielding a maximum activity of 3.2 U/mL and an economic yield of 8.11 ? of MTG/? of nutrients. The results showed that the medium milk-potato-glycerol was feasible for the production of transglutaminase, obtaining a high MTG activity in a simple natural medium.
机译：这项工作的目的是评估微生物转谷氨酰胺酶(MTG)的生产链霉菌属mobaraensis减少营养基于非商业土豆培养基。最初,生产比较研究了3文化媒体基于土豆:(i)媒体的基础上酸水解马铃薯,(2)媒体的基础上gelified non-hydrolysed土豆和(iii)媒体基于gelified non-hydrolysed土豆补充葡萄糖(20 g / L)。补充营养和电感的MTG生产(酵母提取物,蛋白胨,钠炮塔、甘油和矿物盐等磷酸钠、磷酸钾、镁硫酸)。non-hydrolysed土豆可以获得2.72 U /毫升96 h。在另一项研究中,提出了替代所有的营养并通过完全脱脂牛奶和电感甘油。优化的复合牛奶,土豆和甘油。也评估。和采集标本72 h和96 h。结果显示最大MTG活动在72 h。土豆和牛奶的显著影响MTG观察活动,包括一个效果这两个化合物之间的相互作用。选择条件:600 g / L的脱脂牛奶,40 g / L的土豆和5 g / L的甘油,屈服的最大活动3.2 U /毫升和经济收益率的8.11 ?结果表明,介质milk-potato-glycerol是可行的转谷氨酰胺酶的生产,获得高MTG活动在一个简单的自然中。

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