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首页> 外文期刊>Journal of Cellular Physiology >DJ-1/Park7 Sensitive Na+/H+ Exchanger 1 (NHE1) in CD4(+) T Cells
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DJ-1/Park7 Sensitive Na+/H+ Exchanger 1 (NHE1) in CD4(+) T Cells

机译：DJ-1 / Park7敏感Na + / H +换热器1 (NHE1)CD4 (+) T细胞

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DJ-I /Park7 is a redox-sensitive chaperone protein counteracting oxidation and presumably contributing to the control of oxidative stress responses and thus inflammation. DJ-I gene deletion exacerbates the progression of Parkinson's disease presumably by augmenting oxidative stress. Formation of reactive oxygen species (ROS) is paralleled by activation of the Na+/H+ exchanger I (NHEI). ROS formation in CD4(+) T cells plays a decisive role in regulating inflammatory responses. In the present study, we explored whether DJ-I is expressed in CD4(+) T cells, and affects ROS production as well as NHEI in those cells. To this end, DJ-I and NHEI transcript, and protein levels were quantified by qRT-PCR and Western blotting, respectively, intracellular pH (pH;) utilizing bis-(2carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) fluorescence, NHE activity from realkalinization after an ammonium pulse, and ROS production utilizing dichlorofluorescin diacetate (DCFDA) fluorescence. As a result DJ-I was expressed in CD4(+) T cells. ROS formation, NHEI transcript levels, NHE I protein, and NHE activity were higher in CD4(+-)T cells from DJ-I deficient mice than in CD4(+) T cells from wild type mice. Antioxidant N-acetyl-cysteine (NAC) and protein tyrosine kinase (PTK) inhibitor staurosporine decreased the NHE activity in DJ-I deficient CD4(+) T cells, and blunted the difference between DJ-I-/- and DJ-I (+/+) CD4(+) T cells, an observation pointing to a role of ROS in the up-regulation of NHEI in DJ-I CD4(+) T cells. In conclusion, DJ-I is a powerful regulator of ROS production as well as NHEI expression and activity in CD4(+) T cells. 2016 Wiley Periodicals, Inc.
机译：DJ-I / Park7 redox-sensitive伴护蛋白质抵消氧化和可能导致氧化应激的控制反应,因此炎症。删除加剧的发展帕金森病可能增加氧化应激。物种(ROS)被激活的平行Na + / H +换热器(NHEI)。CD4 (+) T细胞起着决定性的作用调节炎症反应。研究中,我们探索DJ-I是否表达CD4 (+) T细胞,并影响ROS生产在这些细胞以及NHEI。NHEI转录和蛋白质含量量化存在和免疫印迹细胞内的pH值(酸碱度;)分别利用之二(2carboxyethyl) - 5 - (and-6 -carboxyfluorescein)(BCECF)荧光,新人道活动realkalinization铵后脉冲和活性氧生产利用dichlorofluorescin二乙酸(DCFDA)荧光。表达CD4 (+) T细胞。转录水平,新人道我蛋白质,和新人道活动高从DJ-I CD4 (+) T细胞有缺陷的小鼠比野生的CD4 (+) T细胞类型老鼠。和蛋白质酪氨酸激酶抑制剂(PTK)在DJ-I staurosporine减少新人道活动缺乏CD4 (+) T细胞,削弱区别DJ-I - / -和DJ-I CD4 (+) (+ / +)T细胞,观察指着活性氧的作用在DJ-I NHEI老年病的CD4 (+) T细胞。监管机构以及NHEI活性氧的生产表达和CD4 (+) T细胞的活动。威利期刊公司。

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