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首页> 外文期刊>Journal of Cellular Physiology >Effects of Sex and Notch Signaling on the Osteocyte Cell Pool
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Effects of Sex and Notch Signaling on the Osteocyte Cell Pool

机译：性和Notch信号的影响骨细胞细胞池

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Osteocytes play a fundamental role in mechanotransduction and skeletal remodeling. Sex is a determinant of skeletal structure, and female C57BL/6J mice have increased osteoblast number in cancellous bone when compared to male mice. Activation of Notch in the skeleton causes profound cell-context dependent changes in skeletal physiology. To determine the impact of sex and of Notch signaling on the osteocyte cell pool, we analyzed cancellous and cortical bone of 1-6-month-old C57BL/6J or 129SvJ/C57BL/6J mice and determined the osteocyte number/area. There was an age-dependent decline in osteocyte number in cancellous bone of male but not female mice, so that 6-month-old female mice had a greater number of osteocytes than male littermates. Although differences between male and female mice were modest, female mice had approximate to 10-15% greater number of osteocytes/area. RNA sequence analysis of osteocyte-rich preparations did not reveal differences between sexes in the expression of genes known to influence bone homeostasis. Neither the activation of Notch1 nor the concomitant inactivation of Notch1 and Notch2 in Osterix (Sp7) or Dentin matrix protein 1 (Dmp1) expressing cells had a pronounced and consistent effect on cancellous or cortical bone osteocyte number in either sex. Moreover, inactivation of Notch1 and Notch2 in Dmp1 expressing cells did not influence the bone loss in a muscle immobilization model of skeletal unloading. In conclusion, cancellous bone osteocytes decline with age in male mice, cortical osteocytes are influenced by sex in younger mice, but osteocyte cell density is not affected substantially by Notch signaling. J. Cell. Physiol. 232: 363-370, 2017. (c) 2016 Wiley Periodicals, Inc.
机译：骨细胞发挥着基础性的作用转导和骨重建。行列式的骨骼结构,女性C57BL / 6 j小鼠成骨细胞增加在松质骨相比,男性老鼠。深刻cell-context依赖的变化骨骼生理机能。性和骨细胞Notch信号的细胞池,我们的皮质骨和松质情况进行分析1-6-month-old C57BL / 6 j或129 svj / C57BL / 6 j小鼠并确定骨细胞数量/区域。是年龄相关性骨细胞数量下降松质骨的男性而不是女性的老鼠,这6个月大的雌性老鼠有一个更大的骨细胞的数量比男性同胞。虽然男性和女性之间的差异老鼠是适度的,雌性老鼠有近似10 - 15%的骨细胞/区域。序列分析的osteocyte-rich准备没有揭示性别差异吗已知基因的表达影响骨体内平衡。随之而来的失活Notch1和Notch2在Osterix (Sp7)或牙本质基质蛋白1(Dmp1)表达细胞有明显的和对松质或皮质骨一致的影响无论男女,骨细胞数量。失活的Notch1和Notch2 Dmp1表达的细胞没有影响骨质流失在肌肉骨骼的固定模式卸载。在雄性老鼠骨细胞随着年龄的增长而下降,皮质骨细胞受到性别的影响年轻的老鼠,但骨细胞细胞密度不是大大影响了Notch信号。细胞。期刊、公司。

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