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首页> 外文期刊>Journal of Cellular Physiology >Osteoclast Fusion: Time-Lapse Reveals Involvement of CD47 and Syncytin-1 at Different Stages of Nuclearity
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Osteoclast Fusion: Time-Lapse Reveals Involvement of CD47 and Syncytin-1 at Different Stages of Nuclearity

机译：破骨细胞融合:延时显示参与CD47和Syncytin-1的不同阶段Nuclearity

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Investigations addressing the molecular keys of osteoclast fusion are primarily based on end-point analyses. No matter if investigations are performed in vivo or in vitro the impact of a given factor is predominantly analyzed by counting the number of multi-nucleated cells, the number of nuclei per multinucleated cell or TRAcP activity. But end-point analyses do not show how the fusion came about. This would not be a problem if fusion of osteoclasts was a random process and occurred by the same molecular mechanism from beginning to end. However, we and others have in the recent period published data suggesting that fusion partners may specifically select each other and that heterogeneity between the partners seems to play a role. Therefore, we set out to directly test the hypothesis that fusion factors have a heterogenic involvement at different stages of nuclearity. Therefore, we have analyzed individual fusion events using time-lapse and antagonists of CD47 and syncytin-1. All time-lapse recordings have been studied by two independent observers. A total of 1808 fusion events were analyzed. The present study shows that CD47 and syncytin-1 have different roles in osteoclast fusion depending on the nuclearity of fusion partners. While CD47 promotes cell fusions involving mono-nucleated pre-osteoclasts, syncytin-1 promotes fusion of two multi-nucleated osteoclasts, but also reduces the number of fusions between mono-nucleated pre-osteoclasts. Furthermore, CD47 seems to mediate fusion mostly through broad contact surfaces between the partners' cell membrane while syncytin-1 mediate fusion through phagocytic-cup like structure. (C) 2016 Wiley Periodicals, Inc.
机译：调查处理的分子键破骨细胞融合主要是基于终点分析。进行体内或体外的影响主要是分析了给定因素计数multi-nucleated细胞的数量,每多核细胞的细胞核数量或TRAcP活动。融合了。如果破骨细胞的融合是一个随机的问题过程和发生同样的分子从头到尾的机制。其他人则在近期公布的数据表明融合伙伴可能特别选择彼此之间的异质性合作伙伴似乎发挥了作用。直接测试的假设融合因素有异型的参与不同的nuclearity阶段。分析了个人融合事件使用延时和CD47和拮抗剂syncytin-1。研究了两个独立的观察者。1808年融合事件进行了分析。研究表明,CD47, syncytin-1不同的角色在破骨细胞融合取决于融合的nuclearity伙伴。促进细胞融合涉及mono-nucleatedpre-osteoclasts, syncytin-1促进融合两个multi-nucleated破骨细胞,但也会降低mono-nucleated之间的融合pre-osteoclasts。协调融合主要通过广泛接触伙伴的细胞膜之间的表面虽然syncytin-1协调融合phagocytic-cup等结构。期刊、公司。

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