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首页> 外文期刊>Journal of Cellular Physiology >Aging Selectively Modulates Vitamin C Transporter Expression Patterns in the Kidney
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Aging Selectively Modulates Vitamin C Transporter Expression Patterns in the Kidney

机译：衰老有选择地调节维生素C运输车肾脏中的表达模式

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In the kidney, vitamin C is reabsorbed from the glomerular ultrafiltrate by sodium-vitamin C cotransporter isoform 1 (SVCT1) located in the brush border membrane of the proximal tubules. Although we know that vitamin C levels decrease with age, the adaptive physiological mechanisms used by the kidney for vitamin C reabsorption during aging remain unknown. In this study, we used an animal model of accelerated senescence (SAMP8 mice) to define the morphological alterations and aging-induced changes in the expression of vitamin C transporters in renal tissue. Aging induced significant morphological changes, such as periglomerular lymphocytic infiltrate and glomerular congestion, in the kidneys of SAMP8 mice, although no increase in collagen deposits was observed using 2-photon microscopy analysis and second harmonic generation. The most characteristic histological alteration was the dilation of intracellular spaces in the basolateral region of proximal tubule epithelial cells. Furthermore, a combination of laser microdissection, qRT-PCR, and immunohistochemical analyses allowed us to determine that SVCT1 expression specifically increased in the proximal tubules from the outer strip of the outer medulla (segment S3) and cortex (segment S2) during aging and that these tubules also express GLUT1. We conclude that aging modulates vitamin C transporter expression and that renal over-expression of SVCT1 enhances vitamin C reabsorption in aged animals that may synthesize less vitamin C. (C) 2016 Wiley Periodicals, Inc.
机译：在肾脏,维生素C的吸收肾小球超滤液通过sodium-vitamin C转运蛋白1 (SVCT1)位于同种型近端小管的刷状缘膜。尽管我们知道,维生素C含量减少随着年龄的增长,自适应的生理机制用维生素C的肾脏重吸收在衰老仍然未知。使用加速衰老的动物模型(SAMP8小鼠)定义形态改变和aging-induced变化维生素C的表达在肾脏转运蛋白组织。变化,如periglomerular淋巴细胞渗透和肾小球充血,SAMP8小鼠的肾脏,虽然没有增加使用2-photon观察胶原沉积显微镜分析和二次谐波的一代。改变是细胞内的扩张空间基底外侧区域的近端小管上皮细胞。激光显微解剖,结合中存在,让我们和免疫组织化学分析确定SVCT1表达明确增加的近端小管外带外的髓质(段S3)皮层(段S2)在衰老和这些小管也表达GLUT1。衰老调节维生素C转运体的表达肾SVCT1的表达增强维生素C在年龄的动物可能再吸收合成维生素C。(C) 2016小威利期刊、公司。

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