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首页> 外文期刊>Journal of Cellular Physiology >TNT-Induced Phagocytosis: Tunneling Nanotubes Mediate the Transfer of Pro-Phagocytic Signals From Apoptotic to Viable Cells
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TNT-Induced Phagocytosis: Tunneling Nanotubes Mediate the Transfer of Pro-Phagocytic Signals From Apoptotic to Viable Cells

机译：TNT-Induced吞噬作用:隧道纳米管调解Pro-Phagocytic信号的转移从可行的细胞凋亡

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The exposure of phosphatidylserine (PS) on the surface membrane of apoptotic cells triggers the recruitment of phagocytic receptors and subsequently results in uptake by phagocytes. Here we describe how apoptotic cells can use intercellular membrane nanotubes to transfer exposed PS to neighboring viable cells, and thus deposit an "eat-me" tag on the viable cells. Tunneling nanotubes (TNTs) connected UV-treated apoptotic rat pheochromocytoma PC12 cells with neighboring untreated cells. These TNTs were composed of PS-exposed plasma membrane and facilitated the transfer of the membrane from apoptotic to viable cells. Other pro-phagocytic signals, such as oxidized phospholipids and calreticulin, were also transferred to viable cells. In addition, anti-phagocytic signal CD47 presenting on the plasma membrane of viable cells was masked by the transferred PS-membrane. Confocal imaging revealed an increase of phagocytosis of viable PC12 cells by murine RAW264.7 macrophages when the viable PC12 cells were cocultured with UV-treated PC12 cells. Treatment with 50 nM cytochalasin D would abolish TNTs and correspondingly inhibit this phagocytosis of the viable cells. Our study indicates that exposed-PS membrane is delivered from apoptotic to viable cells through TNTs. This transferred membrane may act as a pro-phagocytic signal for macrophages to induce phagocytosis of viable cells in a situation where they are in the vicinity of apoptotic cells. (C) 2016 The Authors. Journal of Cellular Physiology Published by Wiley Periodicals Inc.
机译：的磷脂酰丝氨酸(PS)表面膜触发凋亡细胞吞噬受体和招聘随后导致吞噬细胞所吸收。在这里,我们描述凋亡细胞如何使用细胞间转移膜纳米管暴露PS邻近可行的细胞,因此存款一个可行的细胞“吃我”的标签。隧道纳米管(tnt) UV-treated相连凋亡大鼠嗜铬细胞瘤PC12细胞邻近的未经处理的细胞。PS-exposed等离子体膜组成促进了转移膜的凋亡的细胞。信号,如氧化磷脂和calreticulin,也转移到可行的细胞。提出可行的细胞的质膜掩盖了PS-membrane转让。共焦成像显示增加了通过小鼠可行的PC12细胞的吞噬作用RAW264.7巨噬细胞时可行的PC12细胞与UV-treated cocultured PC12细胞。治疗50纳米细胞松弛素D将废除tnt和相应的抑制可行的细胞的吞噬作用。表明exposed-PS膜通过tnt可行的细胞凋亡。转移膜可以作为pro-phagocytic信号诱导巨噬细胞吞噬作用的可行的细胞的情况附近的凋亡细胞。作者。由威利期刊。

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