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首页> 外文期刊>Journal of Cellular Physiology >ATP Release and P2Y Receptor Signaling are Essential for Keratinocyte Galvanotaxis
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ATP Release and P2Y Receptor Signaling are Essential for Keratinocyte Galvanotaxis

机译：ATP释放和P2Y受体信号必不可少的角化细胞趋电性

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Repair to damaged tissue requires directional cell migration to heal the wound. Immediately upon wounding an electrical guidance cue is created with the cathode of the electric field (EF) located at the center of the wound. Previous research has demonstrated directional migration of keratinocytes toward the cathode when an EF of physiological strength (100-150mV/mm) is applied in vitro, but the sensor by which keratinocytes sense the EF remains elusive. Here we use a customized chamber design to facilitate the application of a direct current (DC) EF of physiological strength (100mV/mm) to keratinocytes whilst pharmacologically modulating the activation of both connexin hemichannels and purinergic receptors to determine their role in EF-mediated directional keratinocyte migration, galvanotaxis. In addition, keratinocytes were exposed to DiSCAC(2)(3) dye to visualize membrane potential changes within the cell upon exposure to the applied DC EF. Here we unveil ATP-medicated mechanisms that underpin the initiation of keratinocyte galvanotaxis. The application of a DC EF of 100mV/mm releases ATP via hemichannels activating a subset of purinergic P2Y receptors, locally, to initiate the directional migration of keratinocytes toward the cathode in vitro, the center of the wound in vivo. The delineation of the mechanisms underpinning galvanotaxis extends our understanding of this endogenous cue and will facilitate the optimization and wider use of EF devices for chronic wound treatment. J. Cell. Physiol. 230: 181-191, 2016. (c) 2015 Wiley Periodicals, Inc.
机译：修复受损组织需要定向细胞迁移到伤口愈合。受伤创建电子制导信号的阴极电场(EF)位于中心的伤口。研究表明定向迁移角化细胞向阴极的EF生理强度(100 - 150 mv /毫米)角化细胞的体外,但传感器感觉EF仍然难以捉摸。室设计促进定制应用直流电(DC) EF生理力量(100 mv /毫米)角化细胞同时药物调节两个联接蛋白的激活hemichannels和purinergic受体来确定他们的角色EF-mediated定向角化细胞迁移,趋电性。暴露于DiSCAC(2)(3)染料可视化膜细胞内的潜在变化曝光应用直流EF。ATP-medicated机制支撑角化细胞开始趋电性。应用直流EF 100 mv /毫米释放ATP通过hemichannels激活的一个子集purinergic P2Y受体,在当地,启动角化细胞的定向迁移的方向阴极体外,伤口的中心vivo支撑趋电性扩展我们的理解这种内源性线索和意志促进EF的优化和更广泛地使用慢性伤口的治疗设备。杂志。230:181 - 191年,2016年。期刊、公司。

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