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首页> 外文期刊>Journal of Cellular Physiology >Involvement of Transient Receptor Potential Cation Channel Vanilloid 1 (TRPV1) in Myoblast Fusion
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Involvement of Transient Receptor Potential Cation Channel Vanilloid 1 (TRPV1) in Myoblast Fusion

机译：瞬时受体电位阳离子的参与通道辣椒1 (TRPV1)在成肌细胞融合

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摘要

The mechanisms that underlie the complex process of muscle regeneration after injury remain unknown. Transient receptor potential cation channel vanilloid 1 (TRPV1) is expressed in several cell types, including skeletal muscle, and is activated by high temperature and by certain molecules secreted during tissue inflammation. Severe inflammation and local temperature perturbations are induced during muscle regeneration, which suggests that TRPV1 might be activated and involved in the process. The aim of this study, was to clarify the role of TRPV1 in the myogenic potential of satellite cells responsible for muscle regeneration. We found that mRNA and protein levels of TRPV1 increased during regeneration after cardiotoxin (CTX)-induced muscle injury in mice. Using isolated mouse satellite cells (i.e., myoblasts), we observed that activation of TRPV1 by its agonist capsaicin (CAP) augmented myogenin protein levels. Whereas CAP did not alter myoblast proliferation, it facilitated myoblast fusion (evaluated using myonucleii number per myotube and fusion index). In contrast, suppression of TRPV1 by siRNA impaired myoblast fusion. Using mice, we also demonstrated that intramuscular injection of CAP facilitated muscle repair after CTX-induced muscle injury. Moreover, we showed that these roles of TRPV1 might be mediated by interleukin-4 and calcium signaling during myoblast fusion. Collectively, these results suggest that TRPV1 underlies normal myogenesis through promotion of myoblast fusion. J. Cell. Physiol. 231: 2275-2285, 2016. (c) 2016 Wiley Periodicals, Inc.
机译：背后的机制复杂的过程肌肉再生受伤后依然存在未知的。通道辣椒1 (TRPV1)表示多种细胞类型,包括骨骼肌,并通过高温和激活某些分子期间分泌组织炎症。温度扰动诱导期间肌肉再生,这表明TRPV1可能会被激活,参与这一过程。本研究的目的是澄清的作用TRPV1在卫星的肌原性的潜力细胞负责肌肉再生。TRPV1的发现信使rna和蛋白质含量增加在心脏毒素后再生在老鼠身上(CTX)全身的肌肉拉伤。孤立的老鼠卫星细胞(即肌母细胞),我们观察到激活TRPV1的辣椒素受体激动剂(CAP) myogenin增强蛋白质的水平。成肌细胞增殖,促进成肌细胞融合(评估使用myonucleii /数量肌管和融合指数)。抑制核TRPV1的成肌细胞受损融合。帽促进肌肉的肌内注射CTX-induced肌肉损伤后修复。我们表明,这些角色的TRPV1由interleukin-4和钙信号在成肌细胞融合。结果表明,TRPV1是正常的肌细胞生成通过促进成肌细胞融合。j .细胞。威利期刊公司。

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