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首页> 外文期刊>Journal of Cellular Physiology >A New In Vitro Co-Culture Model Using Magnetic Force-Based Nanotechnology
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A New In Vitro Co-Culture Model Using Magnetic Force-Based Nanotechnology

机译：一个新的使用磁体外共培养模型部队纳米技术

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Skeletal myoblast (SkMB) transplantation has been conducted as a therapeutic strategy for severe heart failure. However, arrhythmogenicity following transplantation remains unsolved. We developed an in vitro model of myoblast transplantation with patterned or randomly-mixed co-culture of SkMBs and cardiomyocytes enabling subsequent electrophysiological, and arrhythmogenic evaluation. SkMBs were magnetically labeled with magnetite nanoparticles and co-cultured with neonatal rat ventricular myocytes (NRVMs) on multi-electrode arrays. SkMBs were patterned by a magnet beneath the arrays. Excitation synchronicity was evaluated by Ca2+ imaging using a gene-encoded Ca2+ indicator, G-CaMP2. In the monoculture of NRVMs (control), conduction was well-organized. In the randomly-mixed co-culture of NRVMs and SkMBs (random group), there was inhomogeneous conduction from multiple origins. In the patterned co-culture where an en bloc SKMB-layer was inserted into the NRVM-layer, excitation homogenously propagated although conduction was distorted by the SkMB-area. The 4-mm distance conduction time (CT) in the random group was significantly longer (197 +/- 126ms) than in control (17 +/- 3ms). In the patterned group, CT through NRVM-area did not change (25 +/- 3ms), although CT through the SkMB-area was significantly longer (132 +/- 77ms). The intervals between spontaneous excitation varied beat-to-beat in the random group, while regular beating was recorded in the control and patterned groups. Synchronized Ca2+ transients of NRVMs were observed in the patterned group, whereas those in the random group were asynchronous. Patterned alignment of SkMBs is feasible with magnetic nanoparticles. Using the novel in vitro model mimicking cell transplantation, it may become possible to predict arrhythmogenicity due to heterogenous cell transplantation. J. Cell. Physiol. 231: 2249-2256, 2016. (c) 2016 Wiley Periodicals, Inc.
机译：骨骼肌成肌细胞移植(SkMB)作为严重的治疗策略心力衰竭。移植后仍没有解决。开发了一个成肌细胞的体外模型移植与图案或随机混合SkMBs和心肌细胞的共培养后续的电生理学,arrhythmogenic评估。磁标以磁铁矿纳米颗粒和培养新生大鼠心室初勘阶段细胞(NRVMs)数组。下面被一块磁铁的数组。励磁同步被Ca2 +评估成像使用gene-encoded Ca2 +指标,G-CaMP2。传导是有序的。随机混合共培养NRVMs SkMBs(随机组),非均匀来自多个来源的传导。的培养,全体SKMB-layer是插入到NRVM-layer,激发组织传播虽然传导由SkMB-area扭曲。传导时间(CT)随机的组大大延长(197 + / - 126 ms)控制(17 + / - 3 ms)。通过NRVM-area没有改变(25 + / - 3 ms),虽然通过SkMB-area CT大大延长(132 + / - 77 ms)。间隔自发激励多样beat-to-beat随机组,而普通殴打被记录在控制和图案组。观察图案组,而这些随机的组是异步的。图案对齐的SkMBs是可行的磁性纳米粒子。模型模拟细胞移植,它可能成为可能的预测arrhythmogenicity由于异质的细胞移植。杂志。231:2249 - 2256年,2016年。期刊、公司。

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