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首页> 外文期刊>Journal of Cellular Physiology >Lack of Dystrophin Affects Bronchial Epithelium in mdx Mice
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Lack of Dystrophin Affects Bronchial Epithelium in mdx Mice

机译：缺乏抗肌萎缩蛋白影响支气管上皮细胞mdx老鼠

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Mild exercise training may positively affect the course of Duchenne Muscular Dystrophy (DMD). Training causes mild bronchial epithelial injury in both humans and mice, but no study assessed the effects of exercise in mdx mice, a well known model of DMD. The airway epithelium was examined in mdx (C57BL/10ScSn-Dmdmdx) mice, and in wild type (WT, C57BL/10ScSc) mice either under sedentary conditions (mdx-SD, WT-SD) or during mild exercise training (mdx-EX, WT-EX). At baseline, and after 30 and 45 days of training (5 d/wk for 6 weeks), epithelial morphology and markers of regeneration, apoptosis, and cellular stress were assessed. The number of goblet cells in bronchial epithelium was much lower in mdx than in WT mice under all conditions. At 30 days, epithelial regeneration (PCNA positive cells) was higher in EX than SD animals in both groups; however, at 45 days, epithelial regeneration decreased in mdx mice irrespective of training, and the percentage of apoptotic (TUNEL positive) cells was higher in mdx-EX than in WT-EX mice. Epithelial expression of HSP60 (marker of stress) progressively decreased, and inversely correlated with epithelial apoptosis (r=-0.66, P=0.01) only in mdx mice. Lack of dystrophin in mdx mice appears associated with defective epithelial differentiation, and transient epithelial regeneration during mild exercise training. Hence, lack of dystrophin might impair repair in bronchial epithelium, with potential clinical consequences in DMD patients. J. Cell. Physiol. 231: 2218-2223, 2016. (c) 2016 Wiley Periodicals, Inc.
机译：温和的运动训练可能会产生积极的影响杜氏肌肉营养不良症(DMD)。训练会导致轻度支气管上皮损伤在人类和老鼠,但是没有研究评估运动的影响在mdx老鼠,一个众所周知的DMD模型。在mdx (C57BL / 10 scsn-dmdmdx)小鼠,在野外类型(WT、C57BL / 10“)在老鼠久坐不动的状况(mdx-SD WT-SD)或期间温和的运动训练(mdx-EX WT-EX)。基线,在30到45天的培训(5d /周6周),上皮形态和标记的再生,细胞凋亡,细胞压力评估。在支气管上皮在mdx低得多在所有条件下比WT老鼠。上皮再生(PCNA阳性细胞)在前两组比SD动物;然而,在45天内,上皮再生减少mdx老鼠无论训练,和凋亡的比例(TUNEL阳性)细胞在mdx-EX高于WT-EX老鼠。上皮的表达HSP60(压力的标志)逐步减少,负相关性与上皮细胞凋亡(r = -0.66, P = 0.01)在mdx老鼠。似乎与上皮缺陷有关分化和瞬态上皮再生过程中轻微的运动训练。因此,缺乏抗肌萎缩蛋白可能损害修复支气管上皮细胞,与潜在的临床DMD患者的后果。231: 2218 - 2223, 2016。公司。

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