...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cellular Physiology >Regulation of Spermatogenic Cell T-Type Ca2+ Currents by Zn2+: Implications in Male Reproductive Physiology
【24h】

Regulation of Spermatogenic Cell T-Type Ca2+ Currents by Zn2+: Implications in Male Reproductive Physiology

机译：精子发生的细胞调节衣架Ca2 +电流通过Zn2 +:影响在男性生殖生理

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Zn2+ is a trace metal which is important for spermatogenesis progression; its deficiency causes atrophy or malignant growth of the testis. Although testis, epididymis, and prostate contain high Zn2+ concentrations, the molecular entities which are modulated by this metal are still under study. Interestingly, spermatogenic cells mainly express Ca(V)3.2-encoded T-type Ca2+ currents (I-CaT) which are positively or negatively modulated by Zn2+ in other tissues. To explore whether I-CaT could be regulated by Zn2+ and albumin, its main physiological carrier, we performed whole cell electrophysiological recordings of spermatogenic cell I-CaT in the absence or presence of different Zn2+ concentrations. Zn2+ decreased I-CaT in a concentration-dependent manner (IC50= 2M) and this inhibition could only be completely removed in presence of albumin. Differently to previous reports, I-CaT did not show a tonic inhibition by Zn2+. Further analysis showed that Zn2+ did not affect the voltage dependency or the kinetics of current activation, but right shifted the steady-state inactivation curve and slowed inactivation and deactivation kinetics. Recovery from inactivation was also altered. However, these apparent alterations in gating properties are not enough to explain the strong I-CaT reduction. Using non-stationary fluctuation analysis, we found that Zn2+ mainly reduced the number of available Ca2+ channels without changing the single channel current amplitude. I-CaT modulation by Zn2+ could be relevant for spontaneous Ca2+ oscillations during spermatogenesis and in pathophysiological conditions such as diabetes. J. Cell. Physiol. 231: 659-667, 2016. (c) 2015 Wiley Periodicals, Inc.
机译：Zn2 +微量金属是重要的精子形成过程;引起睾丸萎缩或恶性增长。尽管睾丸、附睾和前列腺癌高Zn2 +浓度,分子实体由这种金属仍在调制研究。表达Ca (3.2 V)编码的衣架Ca2 +电流(I-CaT)是积极的还是消极的Zn2 +调制的其他组织。I-CaT能否被Zn2 +和监管白蛋白,其主要生理载体,我们执行完整的细胞电生理精子发生的细胞I-CaT的录音缺乏或存在不同Zn2 +浓度。浓度(IC50 = 2米)和这只能抑制完全移除白蛋白的存在。报道,I-CaT没有显示紧张性抑制Zn2 +。影响电压依赖的动力学当前的激活,但改变了稳态失活曲线和放缓失活,失活动力学。从失活也改变了。这些明显的改变控制属性并不足以解释I-CaT强吗减少。分析,我们发现Zn2 +主要是减少了数量的可用Ca2 +渠道单通道电流幅值变化。I-CaT调制通过Zn2 +可能是相关的自发的Ca2 +振荡期间精子发生和病理生理条件如糖尿病。231: 659 - 667, 2016。公司。

著录项

相似文献

  • 外文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号