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首页> 外文期刊>Journal of Cellular Physiology >BMP-2 Induced Expression of PLC1 That is a Positive Regulator of Osteoblast Differentiation
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BMP-2 Induced Expression of PLC1 That is a Positive Regulator of Osteoblast Differentiation

机译：BMP-2 PLC1的诱导表达积极的监管机构的成骨细胞分化

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Bone morphogenetic protein 2 (BMP-2) is a critical growth factor that directs osteoblast differentiation and bone formation. Phosphoinositide-phospholipase C 1 (PLC1) plays a crucial role in the initiation of the genetic program responsible for muscle differentiation. Differentiation of C2C12 mouse myoblasts in response to insulin stimulation is characterized by a marked increase in nuclear PLC1. Here, the function of PLC1 in the osteogenic differentiation was investigated. Briefly, in C2C12 cells treated with BMP-2 we assist to a remarkable increase in PLC1 protein and mRNA expression. The data regarding the influence on differentiation demonstrated that PLC1 promotes osteogenic differentiation by up-regulating alkaline phosphatase (ALP). Moreover, PLC1 is present in the nuclear compartment of these cells and overexpression of a cytosolic-PLC1mutant (cyt-PLC1), which lacks a nuclear localization sequence, prevented the differentiation of C2C12 cells into osteocytes. Recent evidence indicates that miRNAs act as important post transcriptional regulators in a large number of processes, including osteoblast differentiation. Since miR-214 is a regulator of Osterix (Osx) which is an osteoblast-specific transcription factor that is needful for osteoblast differentiation and bone formation, we further investigated whether PLC1 could be a potential target of miR-214 in the control of osteogenic differentiation by gain- and loss- of function experiment. The results indicated that inhibition of miR-214 in C2C12 cells significantly enhances the protein level of PLC1 and promotes C2C12 BMP-2-induced osteogenesis by targeting PLC1. J. Cell. Physiol. 231: 623-629, 2016. (c) 2015 Wiley Periodicals, Inc.
机译：骨形成蛋白2 (BMP-2)是至关重要的生长因子引导成骨细胞分化和骨形成。Phosphoinositide-phospholipase C 1 (PLC1)扮演在基因的启动至关重要的作用项目负责肌肉分化。C2C12小鼠成肌细胞的分化胰岛素刺激的特点是在核PLC1显著增加。在成骨的PLC1的函数分化研究。C2C12细胞BMP-2我们协助处理PLC1显著增加蛋白质和信使rna表达式。分化证明PLC1促进成骨分化的调控碱性磷酸酶(ALP)。在这些细胞的核间cytosolic-PLC1mutant和超表达(cyt-PLC1)缺乏核本地化序列,避免C2C12分化细胞在骨细胞。microrna作为重要的转录监管机构在大量的过程中,包括成骨细胞分化。mir - 214是一个监管机构的Osterix (Osx)一个osteoblast-specific转录因子成骨细胞分化和必要的吗骨形成,我们进一步调查是否PLC1 mir - 214的可能是一个潜在的目标成骨分化的控制增益和损失函数的实验。结果表明,抑制mir - 214C2C12细胞显著增强蛋白质PLC1水平和促进C2C12 BMP-2-induced通过针对PLC1骨生成。231: 623 - 629, 2016。公司。

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