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Osteogenic Potential of Human Oral-Periosteal Cells (PCs) Isolated From Different Oral Origin: An In Vitro Study

机译:人类Oral-Periosteal成骨的潜力细胞(pc)分离出不同的口腔来源:体外研究

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The periosteum is a specialized connective tissue containing multipotent stem cells capable of bone formation. In this study, we aimed at demonstrating that human oral periosteal cells derived from three different oral sites (upper vestibule, lower vestibule, and hard palate) represent an innovative cell source for maxillo-facial tissue engineering applications in terms of accessibility and self-commitment towards osteogenic lineage. Periosteal cells (PCs) were isolated from patients with different ages (20-30 yy, 40-50 yy, 50-60 yy); we then analyzed the in vitro proliferation capacity and the bone self-commitment of cell clones culturing them without any osteogenic supplement to support their differentiation. We found that oral PCs, independently of their origin and age of patients, are mesenchymal stem cells with stem cell characteristics (clonogenical and proliferative activity) and that, even in absence of any osteogenic induction, they undertake the osteoblast lineage after 45 days of culture. These results suggest that oral periosteal cells could replace mesenchymal cells from bone marrow in oral tissue-engineering applications. J. Cell. Physiol. 231: 607-612, 2016. (c) 2015 Wiley Periodicals, Inc.
机译:骨膜是专门的结缔组织包含多功能干细胞骨的能力形成。证明人类口腔骨膜细胞来自三个不同的口语网站(上前庭,降低前庭和硬腭)代表了一种创新的细胞来源maxillo-facial组织工程应用可访问性和自我承诺向成骨的血统。(pc)分离出不同的患者年龄(20 - 30 yy, 40 - 50 yy, 50 - 60 yy);分析了体外增殖能力和骨头自我承诺的细胞克隆培养他们没有任何成骨的补充支持他们的区别。独立的起源和年龄患者,与抑制间充质干细胞(clonogenical和细胞特征增殖活动),即使在缺席任何成骨的感应,他们承担成骨细胞的文化血统后45天。这些结果表明,口服骨膜细胞可以替代从骨髓间充质细胞吗在口腔组织工程的应用。杂志。231:607 - 612年,2016年。期刊、公司。

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