...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cellular Physiology >Prolyl Endopeptidase (PREP) is Associated With Male Reproductive Functions and Gamete Physiology in Mice
【24h】

Prolyl Endopeptidase (PREP) is Associated With Male Reproductive Functions and Gamete Physiology in Mice

机译：Prolyl肽链内切酶(预科)有关男性生殖功能和配子生理机能在老鼠身上

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Prolyl endopeptidase (PREP) is a serine protease which has been implicated in many biological processes, such as the maturation and degradation of peptide hormones and neuropeptides, learning and memory, cell proliferation and differentiation, and glucose metabolism. A small number of reports have also suggested PREP participation in both male and female reproduction-associated processes. In the present work, we examined PREP distribution in male germ cells and studied the effects of its knockdown (Prep(gt/gt)) on testis and sperm in adult mice. The protein is expressed and localized in elongating spermatids and luminal spermatozoa of wild type (wt) mice, as well as Sertoli, Leydig, and peritubular cells. PREP is also expressed in the head and midpiece of epididymal spermatozoa, whereas the remaining tail region shows a weaker signal. Furthermore, testis weight, histology of seminiferous tubules, and epididymal sperm parameters were assessed in wt and Prep(gt/gt) mice: wild type testes have larger average tubule and lumen diameter; in addition, lumenal composition of seminiferous tubules is dissimilar between wt and Prep(gt/gt), as the percentage of spermiated tubules is much higher in wt. Finally, total sperm count, sperm motility, and normal morphology are also higher in wt than in Prep(gt/gt). These results show for the first time that the expression of PREP could be necessary for a correct reproductive function, and suggest that the enzyme may play a role in mouse spermatogenesis and sperm physiology. J. Cell. Physiol. 231: 551-557, 2016. (c) 2015 Wiley Periodicals, Inc.
机译：Prolyl肽链内切酶(预科)是一种丝氨酸蛋白酶已与许多生物吗过程,如成熟和退化肽激素和神经肽,学习和记忆,细胞增殖和分化和葡萄糖代谢。报告还建议准备男性和女性的参与reproduction-associated流程。检查工作,我们准备在男性生殖分配细胞和击倒的影响进行了研究(预科(gt / gt)在成年小鼠睾丸和精子。蛋白质表达和本地化延伸精子和腔的精子野生型小鼠(wt),以及滋养,睾丸间质,和小管细胞。附睾的精子的头部和中段,而剩下的尾巴地区显示较弱信号。细精管,附睾的精子参数在wt评估和准备(gt / gt)老鼠:野生型睾丸有更大的平均小管和内腔直径;细精管构成是不同的wt和预科(gt / gt)之间的比例spermiated小管在wt更高。最后,总精子数、精子的运动性和正常在wt比形态也更高预备(gt / gt)。时间准备的表达需要一个正确的生殖功能,,表明酶发挥作用小鼠精子发生和精子生理机能。细胞。期刊、公司。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号