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首页> 外文期刊>Journal of Cellular Physiology >Mesenchymal Stromal Cells Differentiating to Adipocytes Accumulate Autophagic Vesicles Instead of Functional Lipid Droplets
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Mesenchymal Stromal Cells Differentiating to Adipocytes Accumulate Autophagic Vesicles Instead of Functional Lipid Droplets

机译：间充质基质细胞分化脂肪细胞自噬囊泡而积累功能性脂滴

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Adult bone marrow mesenchymal stromal cells (BMSCs) can easily be differentiated into a variety of cells. In vivo transplantation of BMSCs differentiated cells has had limited success, suggesting that these cells may not be fully compatible with the cells they are intended to replace in vivo. We investigated the structural and functional features of BMSCs-derived adipocytes as compared with adipocytes from adipose tissue, and the structure and functionality of lipid vesicles formed during BMSCs differentiation to adipocytes. Gas chromatography-mass spectrometry showed fatty acid composition of BMSCs-derived adipocytes and adipocytes from the adipose tissue to be very different, as is the lipid rafts composition, caveolin-1 expression, caveolae distribution in their membranes, and the pattern of expression of fatty acid elongases. Confocal microscopy confirmed the absence from BMSCs-derived adipocytes of markers of lipid droplets. BMSCs-derived adipocytes cannot convert deuterated glucose into deuterated species of fatty acids and cannot uptake the deuterated fatty acid-bovine serum albumin complexes from the culture medium, suggesting that intra-cellular accumulation of lipids does not occur by lipogenesis. We noted that BMSCs differentiation to adipocytes is accompanied by an increase in autophagy. Autophagic vesicles accumulate in the cytoplasm of BMSCs-derived adipocytes and their size and distribution resembles that of Nile Red-stained lipid vesicles. Stimulation of autophagy in BMSCs triggers the intra-cellular accumulation of lipids, while inhibition of autophagy prevents this accumulation. In conclusion, differentiation of BMSCs-derived adipocytes leads to intra-cellular accumulation of autophagic vesicles rather than functional lipid droplets, suggesting that these cells are not authentic adipocytes. (C) 2015 Wiley Periodicals, Inc.
机译：成人骨髓间充质基质细胞(综合)可以很容易地分化成一个各种各样的细胞。bmsc分化细胞已经有限成功,这表明这些细胞可能不是完全兼容的细胞替换体内。结构和功能的特点相比之下,BMSCs-derived脂肪细胞从脂肪组织,脂肪细胞和结构和脂质囊泡形成的功能bmsc脂肪细胞的分化。相色谱-光谱法显示脂肪酸的合成BMSCs-derived脂肪细胞脂肪细胞脂肪组织非常不同,脂质筏组成,caveolin-1表情,小窝分布他们的膜,表达的模式脂肪酸elongases。证实了缺席BMSCs-derived脂肪细胞脂滴的标记。BMSCs-derived脂肪细胞不能转换氘葡萄糖的氘物种脂肪酸和不能吸收氘脂肪acid-bovine血清白蛋白复合物培养基,暗示胞内脂质积累没有发生在脂肪生成。脂肪细胞的分化是伴随着自噬的增加。BMSCs-derived堆积在细胞质中脂肪细胞和它们的大小和分布类似于尼罗河红点的脂质囊泡。触发胞内积累的脂质,抑制自噬可以防止这种积累。BMSCs-derived脂肪细胞导致的胞内积累的自噬囊泡而非功能性脂滴,表明这些细胞不是真实的脂肪细胞。

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