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首页> 外文期刊>Journal of Cellular Physiology >Cathepsin K Deficiency Suppresses Disuse-Induced Bone Loss
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Cathepsin K Deficiency Suppresses Disuse-Induced Bone Loss

机译：组织蛋白酶K缺乏抑制因缺乏骨质流失

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摘要

Unloading induces bone loss and causes disuse osteoporosis. However, the mechanism underlying disuse osteoporosis is still incompletely understood. Here, we examined the effects of cathepsin K (CatK) deficiency on disuse osteoporosis induced by using sciatic neurectomy (Nx) model. After 4 weeks of surgery, CatK KO and WT mice were sacrificed and subjected to analyses. For cancellous bone rich region, Nx reduced the bone mineral density (BMD) compared to the BMD in the sham operated side in wild type mice. In contrast, CatK deficiency suppressed such Nx-induced reduction of BMD in cancellous bone. Nx also reduced BMD in the mid shaft cortical bone compared to the BMD in the corresponding region on the sham operated side in wild type mice. In contrast, CatK deficiency suppressed such Nx-induced reduction of BMD in the mid shaft cortical bone. Bone volume (BV/TV) was reduced by Nx in WT mice. In contrast, Cat-K deficiency suppressed such reduction in bone volume. Interestingly, CatK deficiency suppressed osteoclast number and osteoclast surface in the Nx side compared to sham side. When bone marrow cells obtained from Nx side femur of CatK-KO mice were cultured, the levels of the calcified area in culture were increased. Further examination of gene expression indicated that Nx suppressed the expression of genes encoding osteoblast-phenotype-related molecules such as Runx2 and alkaline phosphatase in WT mice. In contrast, CatK deficiency suppressed such reduction. These data indicate that CatK is involved in the disuse-induced bone mass reduction. J. Cell. Physiol. 231: 1163-1170, 2016. (c) 2015 Wiley Periodicals, Inc.
机译：卸载诱发骨质疏松和停止使用骨质疏松症。停止使用骨质疏松症仍然是不完全的理解。组织蛋白酶K (CatK)停止使用不足骨质疏松症引起的坐骨神经切除术(Nx)模型。WT老鼠牺牲和接受分析。减少了骨矿物质密度(BMD)比较BMD的骗局在野生型操作老鼠。这样减少Nx-induced松质骨密度骨头。皮质骨的BMD相应的地区虚假的操作方面野生型老鼠。抑制这种Nx-induced BMD的减少中期轴皮质骨。降低了Nx WT老鼠。缺乏抑制减少这样的骨头体积。破骨细胞和破骨细胞表面的数量Nx的一面而虚假的一面。细胞从Nx侧股骨CatK-KO老鼠是培养,钙化区域的水平在文化增加了。基因表达表明Nx镇压了表达的基因编码osteoblast-phenotype-related分子如Runx2和碱性磷酸酶WT老鼠。相反,CatK缺乏抑制减少。参与因骨量减少。2016.

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