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首页> 外文期刊>Journal of Cellular Physiology >Evidences of Biological Functions of Biliverdin Reductase A in the Bovine Epididymis
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Evidences of Biological Functions of Biliverdin Reductase A in the Bovine Epididymis

机译：证据胆绿素的生物功能还原酶在牛附睾

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摘要

Epididymal sperm binding protein 1 (ELSPBP1) is secreted by the epididymal epithelium via epididymosomes and is specifically transferred to dead spermatozoa during epididymal transit. We identified biliverdin reductase A (BLVRA) as a partner of ELSPBP1 by immunoprecipitation followed by tandem mass spectrometry. Pull down assays showed that these two proteins interact in the presence of zinc ions. The BLVRA enzyme is known to convert biliverdin to bilirubin, both of which possess antioxidant activity. Assessment by real-time RT-PCR showed that BLVRA is highly expressed in the caput and the corpus epididymis, but is expressed at lower levels in the testis and the cauda epididymis. It is primarily found in the soluble fraction of the caput epididymal fluid, is barely detectable in the cauda fluid, and is detectable to a lesser extent in the epididymosome fraction of both caput and cauda fluids. Immunocytometry on epididymal sperm showed that BLVRA is found on all sperm recovered from the caput region, whereas it is undetectable on cauda sperm. Biliverdin and bilirubin are found in higher concentrations in the caput epididymal fluid, as measured by mass spectrometry. Lipid peroxidation was limited by 1M of biliverdin, but not bilirubin when caput spermatozoa were challenged with 500M H2O2. Since immature spermatozoa are a source of reactive oxygen species, BLVRA may be involved in the protection of maturing spermatozoa. It is also plausible that BLVRA is implicated in haemic protein catabolism in the epididymal luminal environment. J. Cell. Physiol. 231: 1077-1089, 2016. (c) 2015 Wiley Periodicals, Inc.
机译：附睾的精子结合蛋白1 (ELSPBP1)附睾的上皮细胞通过分泌的epididymosomes专门转移死精子在附睾的交通。胆绿素还原酶(BLVRA)作为标识合伙人ELSPBP1免疫沉淀反应其次是串联质谱分析。分析表明,这两种蛋白质的交互锌离子的存在。已知胆绿素转换为胆红素,具有抗氧化活性。实时rt - pcr表明BLVRA高度用头和语料库附睾表达,但表达较低水平的睾丸和尾附睾。可溶部分的章附睾的液体,几乎没有尾液中检测到,并在一定程度上是可以探测epididymosome分数的头和尾液体。表明BLVRA发现精子恢复头的地区,而这是无法觉察的在尾精子。章中含量高附睾的液体,以质量谱分析。1 m胆绿素,但不是头时,胆红素精子与500过氧化氢的挑战。不成熟精子的活性氧物种,BLVRA可能参与成熟精子的保护。似是而非的BLVRA与血的蛋白质分解代谢的附睾腔的环境。2016.

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