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首页> 外文期刊>Journal of Cellular Physiology >Light/Dark Environmental Cycle Imposes a Daily Profile in the Expression of microRNAs in Rat CD133(+) Cells
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Light/Dark Environmental Cycle Imposes a Daily Profile in the Expression of microRNAs in Rat CD133(+) Cells

机译：光明/黑暗环境周期规定每天概要文件在老鼠的microrna的表达CD133(+)细胞

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摘要

The phenotype of primary cells in culture varies according to the donor environmental condition. We recently showed that the time of the day imposes a molecular program linked to the inflammatory response that is heritable in culture. Here we investigated whether microRNAs (miRNAs) would show differential expression according to the time when cells were obtained, namely daytime or nighttime. Cells obtained from explants of cremaster muscle and cultivated until confluence (similar to 20 days) presented high CD133 expression. Global miRNA expression analysis was performed through deep sequencing in order to compare both cultured cells. A total of 504 mature miRNAs were identified, with a specific miRNA signature being associated to the light versus dark phase of a circadian cycle. miR-1249 and miR-129-2-3p were highly expressed in daytime cells, while miR-182, miR-96-5p, miR-146a-3p, miR-146a-5p, and miR-223-3p were highly expressed in nighttime cells. Nighttime cells are regulated for programs involved in cell processes and development, as well as in the inflammation, cell differentiation and maturation; while daytime cells express miRNAs that control stemness and cytoskeleton remodeling. In summary, the time of the day imposes a differential profile regarding to miRNA signature on CD133(+) cells in culture. Understanding this daily profile in the phenotype of cultured cells is highly relevant for clinical outputs, including cellular therapy approaches. (C) 2016 Wiley Periodicals, Inc.
机译：主细胞的表型在文化各不相同根据捐赠环境条件。我们最近显示,一天的时间强加一个分子程序有关炎症反应是可遗传的文化。(microrna)将显示微分表达式根据细胞的时候,即白天或夜间。提睾肌外植体和培养,直到融合(类似于20天)高CD133的表达。通过深度测序分析为了比较两种培养细胞。504年成熟的microrna被确定,特定的microrna的签名联系在一起的光明与黑暗阶段的生理周期。mir - 1249和mir - 129 - 2 - 3 - p高度表达在白天的细胞,而mir - 182, mir - 96 - 5 - p,mir - 146 - a - 3 - p, mir - 146 - 5 - p和mir - 223 - 3 - p夜间细胞中高度表达。细胞是调节项目参与细胞流程和开发,以及炎症、细胞分化和成熟;控制具备干细胞和细胞骨架重构。强加一个微分概要文件对于microrna文化上的签名CD133(+)细胞。理解这种日常档案的表型培养细胞的高度与临床相关输出,包括细胞治疗方法。(C) 2016年威利期刊公司。

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