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首页> 外文期刊>Journal of Cellular Physiology >Cell-Autonomous Brown-Like Adipogenesis of Preadipocytes From Retinoblastoma Haploinsufficient Mice
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Cell-Autonomous Brown-Like Adipogenesis of Preadipocytes From Retinoblastoma Haploinsufficient Mice

机译：细胞自动则脂肪形成的Preadipocytes从视网膜母细胞瘤Haploinsufficient小鼠

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Mechanisms behind the emergence of brown adipocyte-like (brite or beige) adipocytes within white adipose tissue (WAT) are of interest. Retinoblastoma protein gene (Rb) haploinsufficiency associates in mice with improved metabolic regulation linked to a greater capacity for fatty acid oxidation and thermogenesis in WAT. We aimed to explain a feasible mechanism of WAT-to-BAT remodeling in this model. Differentiated primary adipocytes and Sca1-positive preadipocytes derived from adipose depots of Rb+/- mice and wild-type siblings were compared. Primary white Rb+/- adipocytes displayed under basal conditions increased glucose uptake and an enhanced expression of brown adipocyte-related genes (Pparg, Ppargc1a, Ppargc1b, Prdm16, Cpt1b) but not of purported beige/brite transcriptional markers (Cd137, Tmem26, Tbx1, Slc27a1, Hoxc9, Shox2). Lack of induction of beige markers phenocopied results in WAT of adult Rb+/- mice. Flow cytometry analysis evidenced an increased number of preadipocytes in WAT depots of Rb+/- mice. Sca1(+) preadipocytes from WAT of Rb+/- mice displayed increased gene expression of several transcription factors common to the brown and beige adipogenic programs (Prdm16, Pparg, Ppargc1a) and of receptors of bone morphogenetic proteins (BMPs); however, among the recently proposed beige markers, only Tbx1 was upregulated. Adult Rb+/- mice had increased circulating levels of BMP7. These results indicate that preadipose cells resident in WAT depots of Rb+/- mice retain an increased capacity for brown-like adipogenesis that appears to be different from beige adipogenesis, and suggest that the contribution of these precursors to the Rb+/- adipose phenotype is driven, at least in part, by interaction with BMP7 pathways. (C) 2016 Wiley Periodicals, Inc.
机译：布朗机制背后的出现adipocyte-like(或米色)脂肪细胞内白色脂肪组织(窟)感兴趣的。视网膜母细胞瘤蛋白基因(Rb)haploinsufficiency associates在老鼠身上代谢调节与更大的改善脂肪酸氧化和能力生热作用在窟。WAT-to-BAT改造的可行的机制这个模型。Sca1-positive preadipocytes来自脂肪仓库的Rb + / -小鼠和野生型的兄弟姐妹比较。显示基底条件下增加葡萄糖吸收和增强表达的布朗adipocyte-related基因(Pparg Ppargc1a,Ppargc1b Prdm16, Cpt1b),但不是传说米色/闪亮转录标记(Cd137感应的米色拟表型标记结果窟的成年Rb + / -老鼠。证明preadipocytes数量增加WAT depots Rb + / -的老鼠。窟的Rb + / -老鼠显示增加的基因几个转录因子的表达常见的棕色和浅褐色脂肪形成的项目(Pparg Prdm16 Ppargc1a)和受体骨形成蛋白(bmp);在最近提议米色标记中,只有Tbx1调节。增加BMP7的循环水平。结果表明,居民preadipose细胞在Rb + / -老鼠窟仓库保持增加能力则出现脂肪形成不同于米色脂肪生成,建议这些前兆的贡献Rb + / -脂肪表型驱动,至少在某种程度上,通过交互BMP7通路。(C) 2016年威利期刊公司。

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