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首页> 外文期刊>Journal of Cellular Physiology >microRNA-151-3p Regulates Slow Muscle Gene Expression by Targeting ATP2a2 in Skeletal Muscle Cells
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microRNA-151-3p Regulates Slow Muscle Gene Expression by Targeting ATP2a2 in Skeletal Muscle Cells

机译：微rna - 151 - 3 - p调节减缓肌肉的基因针对骨骼肌ATP2a2表达式细胞

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摘要

MicroRNAs (miRNAs) are a group of small noncoding RNAs that regulate the stability or translation of cognate mRNAs at the post-transcriptional level. Accumulating evidence indicates that miRNAs play important roles in many aspects of muscle function, including muscle growth and development, regeneration, contractility, and muscle fiber type plasticity. In the current study, we examined the function of miR-151-3p in myoblast proliferation and differentiation. Results show that overexpression of miR-151-3p not only upregulates myoblast proliferation, but also decreases slow muscle gene expression (such as MHC-/slow and slow muscle troponin I) in both C2C12 myotubes and in primary cultures. Alternatively, inhibition of miR-151-3p by antisense RNA was found to upregulate MHC-/slow expression, indicating that miR-151-3p plays a role in muscle fiber type determination. Further investigation into the underlying mechanisms revealed for the first time that miR-151-3p directly targets ATP2a2, a gene encoding for a slow skeletal and cardiac muscle specific Ca2+ ATPase, SERCA2 thus downregulating slow muscle gene expression. Mechanisms by which the alteration in SERCA2 expression induces changes in other slow muscle gene expression levels needs to be defined in future research. J. Cell. Physiol. 230: 1003-1012, 2015. (c) 2014 Wiley Periodicals, Inc., A Wiley Company
机译：小分子核糖核酸(microrna)是一组小非编码rna调节稳定或翻译同源的mrna转录后的水平。microrna在许多方面发挥着重要作用肌肉功能,包括肌肉增长和开发、再生、收缩性和肌纤维类型可塑性。研究中,我们调查了mir - 151 - 3 - p的函数成肌细胞增殖和分化。结果表明,超表达mir - 151 - 3 - p不仅移植成肌细胞增殖,但是也能减少肌肉基因表达(缓慢MHC - /缓慢而减缓肌肉肌钙蛋白I)在两种C2C12肌管和小学文化。另外,抑制mir - 151 - 3 - p反义RNA被发现移植MHC - /慢表达式,表明mir - 151 - 3 - p扮演作用在肌肉纤维类型的决心。调查潜在的机制首次披露,mir - 151 - 3 - pATP2a2直接目标,一个基因编码的减缓骨骼和心脏肌肉特定Ca2 +腺苷三磷酸酶,SERCA2因此下调减缓肌肉基因的表达。变更SERCA2表达引起的变化在其他减缓肌肉基因表达水平的需要在未来的研究被定义。杂志。230:1003 - 1012年,2015年。期刊,Inc .威利公司

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