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首页> 外文期刊>Journal of Cellular Physiology >Hepatic Regenerative Potential of Mouse Bone Marrow Very Small Embryonic-Like Stem Cells
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Hepatic Regenerative Potential of Mouse Bone Marrow Very Small Embryonic-Like Stem Cells

机译：小鼠肝再生潜力的骨头骨髓很小的类似胚胎干细胞

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Very small embryonic-like stem cells (VSELs) are a Sca-1(+)Lin(-)CD45(-) cell population that has been isolated from the bone marrow of mice. The similarities and differences between the mRNA profiles of VSELs and embryonic stem (ES) cells have not yet been defined. Here, we report the whole genome gene expression profile of VSELs and ES cells. We analyzed the global gene expression of VSELs and compared it with ES cells by microarray analysis. We observed that 9,521 genes are expressed in both VSELs and ES cells, 1,159 genes are expressed uniquely in VSELs, and 420 genes are expressed uniquely in ES cells. We found that although VSELs are similar to ES cells in their expression of genes associated with stem cell behavior and pluripotency, there are also differences in their mRNA expression. We further analyzed the expression of stem cell-associated genes in VSELs and ES cells, and found that there were differences in these genes. For instance, the Pkd2 and Yap1 gene were reduced in their expression in VSELs when compared with ES cells. But we also found Zfp54 gene expression was higher in VSELs compared with ES cells. More interestingly, we demonstrated that VSELs express c-kit, the stem cell factor (SCF) receptor. In vitro, SCF promoted VSEL differentiation into hepatic colonies in the presence of hepatocyte growth factor. In vivo, transplantation of VSELs directly into CCl4-induced injured livers significantly reduced serum ALT and AST levels. Therefore, these data suggest that VSELs play a role in the repair of injured livers. J. Cell. Physiol. 230: 1852-1861, 2015. (c) 2014 Wiley Periodicals, Inc.
机译：非常小的类似胚胎干细胞(VSELs)本来林(+)(-)CD45(-)细胞的人群从小鼠的骨髓被隔离。信使rna之间的异同概要VSELs和胚胎干细胞(ES)细胞尚未定义。VSELs和全基因组基因表达谱ES细胞。VSELs和它与胚胎干细胞相比微阵列分析。表达VSELs和ES细胞,1159基因表达在VSELs独特,和420年在胚胎干细胞基因表达独特。发现,尽管VSELs类似于胚胎干细胞与干细胞相关的基因的表达细胞行为和多能性,也有他们的mRNA表达的差异。分析了阀杆细胞相关的表达基因VSELs和ES细胞,发现那里在这些基因差异。Pkd2和Yap1基因都在减少表达VSELs与胚胎干细胞相比。但我们也发现Zfp54基因表达与胚胎干细胞相比,VSELs更高。有趣的是,我们证明了VSELs表达c - kit,干细胞因子(SCF)受体。体外,自洽场提升VSEL分化肝殖民地在肝细胞的存在生长因子。直接进入CCl4-induced受伤的肝脏显著降低血清ALT和AST水平。因此,这些数据表明,VSELs发挥在受伤的肝脏的修复作用。杂志。230:1852 - 1861年,2015年。期刊、公司。

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