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首页> 外文期刊>Journal of Cellular Physiology >A Role for the Chemokine Receptor CCR6 in Mammalian Sperm Motility and Chemotaxis
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A Role for the Chemokine Receptor CCR6 in Mammalian Sperm Motility and Chemotaxis

机译：趋化因子受体CCR6的作用哺乳动物精子能动性和趋化性

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Although recent evidence indicates that several chemokines and defensins, well-known as Inflammatory mediators, are expressed in the male and female reproductive tracts, the location and functional significance of chemokine networks in sperm physiology and sperm reproductive tract interactions are poorly understood. To address this deficiency in our knowledge, we examined the expression and function in sperm of CCR6, a receptor common to several chemoattractant peptides, and screened several reproductive tract fluids for the presence of specific ligands. CCR6 protein is present in mouse and human sperm and mainly localized in the sperm tail with other minor patterns in sperm from mice (neck and acrosomal region) and men (neck and midpiece regions). As expected from the protein immunoblotting and immunofiuorescence results, mouse Ccr6 mRNA is expressed in the testis. Furthermore, the Defb29 mRNA encoding the CCR6 ligand, beta-defensin DEFB29, is expressed at high levels in the epididymis. As determined by protein chip analysis, several chemokines (including some that act through CCR6, such as CCL20/MtP-3alpha (formerly macrophage inflammatory protein 3a) and protein hormones were present in human follicular fluid, endometrial secretions, and seminal plasma. In functional chemotaxis assays, capacitated human sperm exhibited a directional movement towards CCL20, and displayed modifications in motility parameters. Our data indicate that chemokine ligand/receptor interactions in the male, and female genital tracts promote sperm motility and chemotaxis under non-inflammatory conditions. Therefore, some of the physiological reactions mediated by CCR6 iigands in male reproduction extend beyond a pro-inflammatory response and might find application in clinical reproduction and/or contraception.
机译：尽管最近的证据表明,一些趋化因子和defensins,众所周知炎症介质,表示在男性和女性生殖道,位置和趋化因子网络的功能意义精子和精子生理生殖系统相互作用知之甚少。这在我们的知识缺乏,我们检查了CCR6的精子中的表达和功能受体常见的几种化学引诱物肽,筛选几个生殖系统液体为特定的配体的存在。蛋白质存在于老鼠和人类精子和主要定位于精子尾部与其他小老鼠的精子(颈部和模式acrosomal地区)和人(颈部和中段地区)。免疫印迹和immunofiuorescence结果,小鼠睾丸Ccr6 mRNA表达。此外,CCR6 Defb29信使rna编码配体,beta-defensin DEFB29表达附睾的高水平。蛋白质芯片分析,几种趋化因子(包括一些通过CCR6,如CCL20 / MtP-3alpha(以前巨噬细胞炎性蛋白3)和蛋白质激素是存在于人类的卵泡液,子宫内膜分泌物和精浆。功能趋化性分析,生产人类精子定向运动对展出CCL20,显示修改的能动性参数。男配体/受体相互作用,女性生殖道促进精子能动性和在非炎症条件下趋化作用。因此,一些生理反应由CCR6 iigands男性生殖促炎症反应和超越可能会发现应用程序在临床生殖和/或避孕。

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