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首页> 外文期刊>Journal of Cellular Physiology >Sub-toxic nicotine concentrations affect extracellular matrix and growth factor signaling gene expressions in human osteoblasts
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Sub-toxic nicotine concentrations affect extracellular matrix and growth factor signaling gene expressions in human osteoblasts

机译：Sub-toxic尼古丁浓度影响细胞外基质和生长因子信号在人类成骨细胞基因表达

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摘要

Exposure to nicotine and other compounds contained in cigarette smoking affects human health. This study examined the effects of exposure to a single or multiple sub-toxic nicotine concentrations on human osteoblasts. Cell growth and expression of genes involved in bone differentiation, extracellular matrix (ECM) metabolism, and growth factor signaling pathways were investigated in nicotine-treated cells compared to untreated cells. Depending on osteoblast concentration and maturation stages, nicotine differently regulated cell growth. Real-time PCR showed regulated expressions of genes expressed by nicotine-treated osteoblasts compared to untreated cells. Among ECM genes, type I collagen was down-regulated and osteonectin was up-regulated in nicotine-treated osteoblasts; similarly, fibroblast growth factor-1 (FGF1) and fibroblast growth factor-2 (FGF2), two members of FGF signaling system, were discordantly modulated; genes involved in osteoblast maturation and differentiation such as alkaline phosphatase (ALP), runt-related transcription factor-2 (RUNX2), and bone sialoprotein (BSP) were over-expressed after drug treatment. Our results show a positive association between nicotine exposure and osteoblast phenotype and illustrate for the first time a mechanism whereby acute or chronic exposure to sub-toxic nicotine concentrations may affect bone formation through the impairment of growth factor signaling system and ECM metabolism.
机译：暴露在尼古丁和其他化合物吸烟会影响人类健康。研究了接触的影响单个或多个sub-toxic尼古丁对人类成骨细胞浓度。并在骨相关基因的表达分化、细胞外基质(ECM)代谢和生长因子信号通路在nicotine-treated细胞了吗未经处理的细胞。成骨细胞浓度和成熟阶段,尼古丁不同调控细胞生长。实时PCR显示监管的表情基因表达的nicotine-treated造骨细胞未经处理的细胞。I型胶原蛋白是衰减和骨粘连蛋白上调nicotine-treated成骨细胞;因子- 1 (FGF1)和纤维母细胞生长因子2(FGF2),两名FGF信号系统,不一致地调制;成骨细胞的成熟和分化等碱性磷酸酶(ALP)、runt-related转录因子2 (RUNX2),和骨头唾液蛋白(BSP) vegf药物之后治疗。尼古丁暴露和之间的联系成骨细胞表型,说明第一一个机制,即急性或慢性接触sub-toxic尼古丁浓度影响骨形成的障碍生长因子信号系统和ECM新陈代谢。

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