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首页> 外文期刊>Journal of Cellular Physiology >Analysis of global changes in gene expression induced by human polynucleotide phosphorylase (hPNPaseold-35)
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Analysis of global changes in gene expression induced by human polynucleotide phosphorylase (hPNPaseold-35)

机译：分析全球基因表达的变化人类多核苷酸磷酸化酶引起的

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摘要

As a strategy to identify gene expression changes affected by human polynucleotide phosphorylase (hPNPaseold-35), we performed gene expression analysis of HeLa cells in which hPNPaseold-35 was overexpressed. The observed changes were then compared to those of HO-1 melanoma cells in which hPNPaseold-35 was stably knocked down. Through this analysis, 90 transcripts, which positively or negatively correlated with hPNPaseold-35 expression, were identified. The majority of these genes were associated with cell communication, cell cycle, and chromosomal organization gene ontology categories. For a number of these genes, the positive or negative correlations with hPNPaseold-35 expression were consistent with transcriptional data extracted from the TCGA (The Cancer Genome Atlas) expression datasets for colon adenocarcinoma (COAD), skin cutaneous melanoma (SKCM), ovarian serous cyst adenocarcinoma (OV), and prostate adenocarcinoma (PRAD). Further analysis comparing the gene expression changes between Ad.hPNPaseold-35 infected HO-1 melanoma cells and HeLa cells overexpressing hPNPaseold-35 under the control of a doxycycline-inducible promoter, revealed global changes in genes involved in cell cycle and mitosis. Overall, this study provides further evidence that hPNPaseold-35 is associated with global changes in cell cycle-associated genes and identifies potential gene targets for future investigation.
机译：作为一个策略来识别基因表达变化受到人类的多核苷酸磷酸化酶的影响(hPNPaseold-35),我们进行基因表达分析hPNPaseold-35的海拉细胞过表达。相比HO-1黑色素瘤细胞中hPNPaseold-35稳定撞倒了。这一分析,90记录,积极或与hPNPaseold-35负相关表达式,确定了。这些基因与细胞有关通信、细胞周期和染色体组织基因本体论类别。这些基因,积极或消极相关性hPNPaseold-35表达式符合转录数据提取从TCGA(癌症基因组图谱)表达数据集对结肠腺癌(COAD),皮肤皮肤黑色素瘤(SKCM),卵巢浆液性囊腺癌(OV)和前列腺癌腺癌(马)。之间的基因表达的变化广告。海拉细胞overexpressing hPNPaseold-35下控制doxycycline-inducible启动子,揭示了全球基因参与细胞的变化周期和有丝分裂。进一步证明hPNPaseold-35相关联细胞cycle-associated与全球变化基因和识别潜在的目标基因未来的调查。

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