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首页> 外文期刊>Journal of Cellular Physiology >Induction of Dopaminergic Neurons From Human Wharton's Jelly Mesenchymal Stem Cell by Forskolin
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Induction of Dopaminergic Neurons From Human Wharton's Jelly Mesenchymal Stem Cell by Forskolin

机译：从人类诱导多巴胺能神经元沃顿商学院的果冻间充质干细胞Forskolin

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摘要

The purpose of this study was to investigate the Wharton's jelly mesenchymal stem cells differentiation ability toward neuronal fate. Human Wharton's jelly mesenchymal stem cells (hWJMSC) have been isolated from human umbilical cord of full-term births and characterized by flow cytometry analysis for their stem mesenchymal properties through specific surface markers expression (CD73, CD90, and CD105). hWJMSC mesodermal lineage differentiation ability and karyotype analysis were assessed. The trans-differentiation of hWJMSC into neural lineage was investigated in presence of forskolin, an agent known to increase the intracellular levels of cAMP. A molecular profile of differentiated hWJMSC was performed by microarray technology which revealed 1,532 statistically significant modulated genes respect to control cells. Most of these genes are mainly involved in functional neuronal signaling pathways and part of them are specifically required for the neuronal dopaminergic induction. The acquisition of the dopaminergic phenotype was evaluated via immunocytochemistry and Western blot analysis revealed the significant induction of Nurr1, NeuroD1, and TH proteins expression in forskolin-induced hWJMSC. Moreover, the treatment with forskolin promoted, in hWJMSC, a strong upregulation of the neurotrophin Trk receptors related to the high release of brain-derived neurotrophic factor. Taken together these findings show that hWJMSC may be represent an optimal therapeutic strategy for neurological diseases.
机译：本研究的目的是调查沃顿商学院的果冻间充质干细胞向神经元分化能力的命运。人类的沃顿商学院的果冻间充质干细胞人类脐(hWJMSC)隔绝绳的足月出生和特征流式细胞术分析茎间充质属性通过特定的表面标记表达式(CD73 CD90、CD105)。hWJMSC中胚层的谱系分化能力和核型分析评估。trans-differentiation hWJMSC到神经在存在的血统了forskolin,代理会增加细胞内水平的阵营。差异化hWJMSC是由微阵列技术,揭示了1532年统计上显著的调节基因的尊重控制细胞。参与功能的神经信号途径和他们是专门的一部分所需的神经元多巴胺能归纳。多巴胺能的收购表型通过免疫细胞化学和西方评估污点分析揭示了重要的感应Nurr1, NeuroD1,蛋白质表达forskolin-induced hWJMSC。forskolin提拔,在hWJMSC,强劲upregulation生成Trk的受体有关释放脑源性高神经营养因子。结果表明,hWJMSC可能代表一个最佳治疗策略的神经疾病。

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