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首页> 外文期刊>Journal of cellular physiology. >Wnt16 is involved in intramembranous ossification and suppresses osteoblast differentiation through the Wnt/β-Catenin pathway
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Wnt16 is involved in intramembranous ossification and suppresses osteoblast differentiation through the Wnt/β-Catenin pathway

机译：Wnt16参与膜内的骨化和抑制成骨细胞分化Wnt /β连环蛋白通路

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摘要

In the course of embryonic development skeletal elements form either through intramembranous or endochondral ossification. Wnt proteins play diverse roles during vertebrate skeletal development. Wnt16 is a key factor in developing long bones, but its exact role in craniofacial bone formation remains unclear. This study was initially undertaken to investigate the expression of Wnt16 during craniofacial bone development in mouse embryos. Wnt16 expression in the osteoid of calvaria, maxilla, and mandible started later than that of ALP and osteocalcin (OCN), but before mineralization of the craniofacial bones, suggesting that Wnt16 is involved in intramembranous ossification in the head. To confirm this, MC3T3-E1 cells were transfected with an adenovirus containing Wnt16 (Ad-Wnt16). Ad-Wnt16 cells showed decreased ALP activity and less mineralized nodule formations compared with control cells. In addition, the mRNA levels of osteogenic markers were reduced. Moreover, Wnt16 activated β-catenin signaling in MC3T3-E1 cells at both transcription and protein levels as shown by a TOPflash luciferase reporter gene assay and western blot analysis. On the other hand, Wnt/β-catenin pathway blockade by Dickkopf 1 abrogated the suppression of mineralization by Wnt16. Our findings suggest that Wnt16 is involved in intramembranous ossification and suppresses osteoblast differentiation through the Wnt/β-catenin pathway.
机译：在胚胎发育的过程中骨骼元素通过膜内的或形式软骨内成骨。不同的角色在脊椎动物骨骼发展。在颅面长骨头,但其确切的作用骨形成尚不清楚。最初进行调查在颅面骨Wnt16的表达小鼠胚胎的发展。头顶的类骨质,上颌和下颌开始得较晚的高山和骨钙蛋白(OCN),但成矿前颅面骨,这表明Wnt16参与膜内的骨化头。用一个包含Wnt16腺病毒转染(Ad-Wnt16)。活动和更少的矿化结节形成相比之下,控制细胞。成骨的标记的mRNA水平降低。此外,Wnt16激活β连环蛋白信号MC3T3-E1细胞转录和蛋白质如图所示,水平TOPflash荧光素酶的记者基因分析和免疫印迹分析。另一方面,Wnt /β连环蛋白通路封锁Dickkopf 1废除压制由Wnt16矿化。Wnt16参与膜内的骨化和抑制成骨细胞分化通过Wnt /β连环蛋白途径。

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