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首页> 外文期刊>Journal of Cellular Physiology >P2X7 receptors in neurohypophysial terminals: Evidence for their role in arginine-vasopressin secretion
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P2X7 receptors in neurohypophysial terminals: Evidence for their role in arginine-vasopressin secretion

机译：P2X7受体在neurohypophysial终端:精氨酸抗利尿激素作用的证据分泌

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摘要

Arginine-vasopressin (AVP) plays a major role in maintaining cardiovascular function and related pathologies. The mechanism involved in its release into the circulation is complex and highly regulated. Recent work has implicated the purinergic receptor, P2X7R, in a role for catecholamine-enhanced AVP release in the rat hypothalamic-neurohypophysial (NH) system. However, the site of P2X7R action in this endocrine system, and whether or not it directly mediates release in secretory neurons have not been determined. We hypothesized that the P2X7R is expressed and mediates AVP release in NH terminals. P2X7R function was first examined by patch-clamp recordings in isolated NH terminals. Results revealed that subpopulations of isolated terminals displayed either high ATP-sensitivity or low ATP-sensitivity, the latter of which was characteristic of the rat P2X7R. Additional recordings showed that terminals showing sensitivity to the P2X7R-selective agonist, BzATP, were further inhibited by P2X7R selective antagonists, AZ10606120 and brilliant blue-G. In confocal micrographs from tissue sections and isolated terminals of the NH P2X7R-immunoreactivity was found to be localized in plasma membranes. Lastly, the role of P2X7R on AVP release was tested. Our results showed that BzATP evoked sustained AVP release in NH terminals, which was inhibited by AZ10606120. Taken together, our data lead us to conclude that the P2X7R is expressed in NH terminals and corroborates its role in AVP secretion.
机译：精氨酸后叶加压素(avon)过程中起着重要作用维护心血管功能和相关病态。释放到循环是复杂的高度管制。P2X7R purinergic受体,一个角色catecholamine-enhanced avon释放的老鼠hypothalamic-neurohypophysial (NH)系统。然而,P2X7R行动的网站内分泌系统,是否直接调节分泌神经元没有释放被确定。表达和调节在NH avon释放吗终端。在隔离膜片箝记录NH终端。结果显示,亚种群的孤立高ATP-sensitivity终端显示或低ATP-sensitivity,后者河鼠P2X7R的特征。录音显示终端显示P2X7R-selective受体激动剂的敏感性,BzATP,进一步抑制P2X7R选择性拮抗剂,AZ10606120和杰出的blue-G。从组织部分和共焦显微图孤立的NH的终端P2X7R-immunoreactivity被发现本地化在等离子体膜。AVP发布测试。BzATP诱发持续在NH avon释放终端,由AZ10606120抑制。综上所述,引导我们得出这样的结论:我们的数据P2X7R NH终端和表达证实了AVP分泌的作用。

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