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首页> 外文期刊>Journal of Cellular Physiology >Osteoactivin induces transdifferentiation of C2C12 myoblasts into osteoblasts
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Osteoactivin induces transdifferentiation of C2C12 myoblasts into osteoblasts

机译：Osteoactivin诱发C2C12分化转移成肌细胞向成骨细胞

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Osteoactivin (OA) is a novel osteogenic factor important for osteoblast differentiation and function. Previous studies showed that OA stimulates matrix mineralization and transcription of osteoblast specific genes required for differentiation. OA plays a role in wound healing and its expression was shown to increase in post fracture calluses. OA expression was reported in muscle as OA is upregulated in cases of denervation and unloading stress. The regulatory mechanisms of OA in muscle and bone have not yet been determined. In this study, we examined whether OA plays a role in transdifferentiation of C2C12 myoblast into osteoblasts. Infected C2C12 with a retroviral vector overexpressing OA under the CMV promoter were able to transdifferentiate from myoblasts into osteoblasts. Immunofluorescence analysis showed that skeletal muscle marker MF-20 was severely downregulated in cells overexpressing OA and contained significantly less myotubes compared to uninfected control. C2C12 myoblasts overexpressing OA showed an increase in expression of bone specific markers such as alkaline phosphatase and alizarin red staining, and also showed an increase in Runx2 protein expression. We also detected increased levels of phosphorylated focal adhesion kinase (FAK) in C2C12 myoblasts overexpressing OA compared to control. Taken together, our results suggest that OA is able to induce transdifferentiation of myoblasts into osteoblasts through increasing levels of phosphorylated FAK. J. Cell. Physiol. 229: 955-966, 2014.
机译：Osteoactivin (OA)是一种新型的成骨的因素对成骨细胞分化和很重要函数。刺激基质矿化,成骨细胞特定基因的转录分化所必需的。伤口愈合及其表达式了增加骨折老茧。据报道在OA肌肉调节去神经和卸载压力。监管机制OA的肌肉和骨骼尚未确定。检查是否OA扮演了一个角色分化转移的C2C12成肌细胞造骨细胞。向量overexpressing OA CMV启动子从肌母细胞能够transdifferentiate吗为成骨细胞。表明骨骼肌MF-20标志在细胞overexpressing OA严重表达下调和含有肌小管明显较低而未受感染的控制。overexpressing OA的增加骨的表达特定的标记等碱性磷酸酶和茜素红染色,同时也显示增加Runx2蛋白质表达式。磷酸化粘着斑激酶(FAK)C2C12成肌细胞overexpressing OA相比控制。OA是能够诱导分化转移通过增加肌母细胞向成骨细胞磷酸化FAK的水平。229: 955-966, 2014.

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