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首页> 外文期刊>Journal of Cellular Physiology >Stress and matrix-responsive cytoskeletal remodeling in fibroblasts
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Stress and matrix-responsive cytoskeletal remodeling in fibroblasts

机译：压力和matrix-responsive细胞骨架装修在成纤维细胞

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摘要

In areolar "loose" connective tissue, fibroblasts remodel their cytoskeleton within minutes in response to static stretch resulting in increased cell body cross-sectional area that relaxes the tissue to a lower state of resting tension. It remains unknown whether the loosely arranged collagen matrix, characteristic of areolar connective tissue, is required for this cytoskeletal response to occur. The purpose of this study was to evaluate cytoskeletal remodeling of fibroblasts in, and dissociated from, areolar and dense connective tissue in response to 2h of static stretch in both native tissue and collagen gels of varying crosslinking. Rheometric testing indicated that the areolar connective tissue had a lower dynamic modulus and was more viscous than the dense connective tissue. In response to stretch, cells within the more compliant areolar connective tissue adopted a large "sheet-like" morphology that was in contrast to the smaller dendritic morphology in the dense connective tissue. By adjusting the in vitro collagen crosslinking, and the resulting dynamic modulus, it was demonstrated that cells dissociated from dense connective tissue are capable of responding when seeded into a compliant matrix, while cells dissociated from areolar connective tissue can lose their ability to respond when their matrix becomes stiffer. This set of experiments indicated stretch-induced fibroblast expansion was dependent on the distinct matrix material properties of areolar connective tissues as opposed to the cells' tissue of origin. These results also suggest that disease and pathological processes with increased crosslinks, such as diabetes and fibrosis, could impair fibroblast responsiveness in connective tissues.
机译：在小区的“宽松”的结缔组织,成纤维细胞在几分钟之内在改造他们的细胞骨架对静态拉伸导致增加胞体横截面积,放松组织到一个较低的静息张力的状态。未知是否松散排列胶原蛋白矩阵,网状的特征结缔组织,是必需的细胞骨架反应发生。本研究旨在评估细胞骨架重构的成纤维细胞,分离蜂窝和致密结缔组织反应2 h的静态伸展在本地组织和不同交联的胶原凝胶。Rheometric测试表明,网状动态模量和结缔组织较低粘性比密集的连接吗组织。更兼容的网状结缔组织一大“片状”形态对比较小的树突形态致密结缔组织。体外胶原蛋白交联,生成的动态模量,证明了细胞分离的致密结缔组织能够响应当播种兼容矩阵,而细胞分离网形结缔组织会失去他们的能力响应矩阵时变得更强硬。这组实验表明stretch-induced成纤维细胞是依赖于扩张不同的矩阵蜂窝材料的性质结缔组织与细胞的组织的起源。与增加疾病和病理过程交联,如糖尿病和纤维化影响成纤维细胞在连接反应组织。

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